Genomic DNA Repository

[Sebastian Cocioba]

I've been searching for some gDNA of ye olde Aquoria Victoria jellyfish and stumbled upon a few sites that sell pure gDNA for like $230. That's ludicrous. Does anyone want to make a decentralized gDNA repo/sharing program?

Just dedicate one tube box to gDNA from organism you work with often and keep them on a list like github for others to request. They just pay shipping. Maybe a buck for the effort?

I have a decent collection of plant gDNA extracted using a simple buffer like the one posted in openwetware.org under "Arabidopsis gDNA". I keep mine in water for short term use and TE for long term storage. Have an Arabidopsis gDNA sample from 2010 and I've managed to amplify all kinds of fragments with no error even recently. Seems stable enough. Wouldn't take long to extract either. What say you all?

Wanna setup a gDNA share program of sorts? Easy way to get PCR-ready gDNA within a week's time.


Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

[Alex]

Sounds like an awesome idea! I don't have anything to contribute yet but I'm sure it's just a matter of time!

Alex

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[EdwardPerello]

Sebastian,

The platform we have developed, AutoClone (which I talk about here - https://groups.google.com/forum/#!topic/diybio/PsMR-zcVffs) would perhaps be a good thing to use to make this happen, as it is pretty much a decentralised DNA repository that supports sharing DNA sequences between multiple users and groups.

If there are plasmids or gDNA that you are willing to share to other people, all you need to do is upload the corresponding "inventory" sequence and make it public. If there are elements within your gDNA that are present in my "designs", then AutoClone would design PCR primers to extract that gDNA, and put you and I in touch for me to arrange shipping from you.

As I mentioned elsewhere, we have a number of iGEM teams using the software this year to share their inventories, and I think it makes good sense for the DIYbio community to share DNA and develop a distributed community repository.

[Koeng]

Sure! How about someone just makes a github and everyone posts the DNA they have there and what they would like in exchange? Maybe like 5 bucks for the effort

[SC]

Hi Sebastian,

What sites did you find that sell pure gDNA? 

Thanks,

Stacy

[Sebastian Cocioba]

http://zyagen.com/index.php?main_page=index&cPath=6_460&zenid=3798c6b92a6d6da2dd3d0f6b2e5db693

One of the sites I recall.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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From: 'SC' via DIYbio
Sent: ‎6/‎27/‎2014 9:25 AM
To: diy...@googlegroups.com
Subject: [DIYbio] Re: Genomic DNA Repository

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[SC]

Neat, thanks!

 

[Koeng]

https://github.com/Koeng101/Biohacking-Plasmids

I made a small list of plasmids I have. I'll try and update it later with sequences or at least links to sequences and clean it up and add some new DNA that I've gotten from people

Anyone want to make an official list?

On Thursday, June 26, 2014 12:14:59 AM UTC-7, Sebastian wrote:

[Dakota Hamill]

I have so much genomic Apple DNA...I could be a millionaire.  

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