DiY Plasmid construction and yeast tranformation

[Jim Wool]

Hello I am starting a new project and it involves taking plasmids with genes that are not present in the yeast genome and transforming them inside. I've been doing some reading and I have the sequences that I intend on using but I was curious if there is a better place to purchase plasmids than the ones on the top of Google results. Also if you folks have any input I would gladly appreciate it.

[Dakota Hamill]

https://www.addgene.org/

I hear many people vouch for them, but I hear as many people say they are miserable to get anything from if you're not a school. 

On Wed, Mar 6, 2019 at 2:25 PM Jim Wool <jimk...@gmail.com> wrote:

Hello I am starting a new project and it involves taking plasmids with genes that are not present in the yeast genome and transforming them inside. I've been doing some reading and I have the sequences that I intend on using but I was curious if there is a better place to purchase plasmids than the ones on the top of Google results. Also if you folks have any input I would gladly appreciate it.

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[Rednaz]

Is there a way to order specific strains of yeast if you have the entire genome? Instead of doing the transformation if it's already out there

[Yoshi Goto]

Addgene hasn't been a problem for our Community Lab, and we're not associated with any school or university.

Not sure where you are, but for yeast strains, there is this: https://nrrl.ncaur.usda.gov/ 

[poli]

The ODIN sells a yeast transformation kit http://www.the-odin.com/ge-yeast/, they are a DIYbio focused company . If you use my coupon code learn there is a 10% discount.