On Tue, Mar 12, 2013 at 3:34 PM, Jeswin <
phill...@gmail.com> wrote:
> On Tue, Mar 12, 2013 at 4:04 PM, Eugen Leitl <
eu...@leitl.org> wrote:
>>
>> EtBr is overdemonized. Many reagents chemists used to work with
>> make it look like weak mouthwash.
>>
>
> If you can't put it in regular waste, then it makes no difference.
> The gov't says "no way" so what are we little guys supposed to do?
>
Could you just throw it in a warm oven to dry it, then wait til you
have a lot and find some company to take it from you?
> I mean, it's a pretty strong DNA intercalator. I only use 1uL EtBr in
> a 50mL gel and get really bright bands. The Gel Red dye says it needs
> 16mL for a 50mL gel. That's a lot of Gel Red compared with EtBr. I
> assume you get the same results. Am I right?
Not really, since the 3X is simply 10000X diluted in dH2O (that's why
they sell both, the former being more expensive but more convenient if
you're.... I'm not sure why you'd buy that one, anyway...)
Also the claimed sensitivity of gelGreen over EtBr is better (they
claim 0.1ug but don't give dimensions mm^2 or mm^3)
--
-Nathan