Record Audio Onset

[zhehao]

Hi,

I am using Bonsai for Fiber Photometry (FP). I would like to present a lot of different audio stimuli (using labview) to animals while recording the FP signals. So I need to know when and what the audio signal is presented.  What I am thinking about now is to split audio output into two. One gose to the speaker, another one goes to the audio interface (connected with the PC). Then how can I use Bonsai to acquire the signals received by the audio interface and sync the time with the FP signal ?

Or is there any other way that I can directly send audio signal to Bonsai directly from labview?

Thank you so much!

Zhehao

[brunocruz]

Hi Zhehao,

By audio interface do you mean microphone? If that is the case I am afraid it wont solve your problem, since it wont guarantee that the mic input is synced with your fiber photometry acquisition system. Does your fiber photometry setup support some sort of extra analog input that you could use to record your audio signal? 

cheers

Bruno

[zhehao]

Hi Bruno,

There is an analog input port in the fiber photometry acquisition system. Maybe I can use it for synchronization. Then I was wondering how should I code it in Bonsai? Maybe Analog-Input module for Ardunio ( the system is ardunio based) ?

Zhehao

[brunocruz]

Hi Zhehao,

What is the system you are currently using?

B

[zhehao]

Hi Bruno,

Neurophotometrics FP3001 is the acquisition system I am using.

Zhehao

[brunocruz]

I am, unfortunately, not familiar with that acquisition system so I am afraid I won't be of much help... I hope someone else is able to help you out!

[zhehao]

No worries, thank you for your help! By the way, I have just posted another post about package installation. I was wondering if you have ideas about it.

[Gonçalo Lopes]

Hi Zhehao,

I believe the FP3001 auxiliary BNC is more for TTL triggering of frames, or for outputting a sync signal, I'm not aware that you can use it as an analog input port. However, going back to your original idea of synching audio in the computer, latencies might be in the order of 10ms but this might be good enough for photometry signals.

You can use the AudioCapture source to grab audio data from either a microphone or line-in input in the soundcard and then combine it with photometry data using CombineLatest or WithLatestFrom. You would need to decide in which format you would like to see the data synchronised, either marking the audio stimulus data with photometry, or the opposite, label the photometry frame with whether there was an onset of a stimulus in that frame.

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