Hi Nancy,
From what you write, it seems that you have no proof that you have a queen in Colony B. Did you look for eggs? You might have a queen "in process" in Colony B and not yet have eggs. Introducing another queen to Colony B would be unwise, if that is the case. You should expect to see eggs 3-4 weeks after the split; 5/25 plus three weeks is 6/15.
If I were you, I'd put the new queen into a nuc with nurse bees until you are sure of the status of Colony B.
Mike
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