Problem after mapping
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Ruchi Choudhary
Dec 1, 2019, 6:10:06 AM12/1/19
to 3D Genomics
Hello NevaI am using Juicer tools for Hi-C analysis and after running the programme i am getting the following error got:
(-: Mem align of /home/Juicer_test_run/splits/HC-1_R2.fastq.gz.sam done successfully
(-: Sort read 1 aligned file by readname completed.
(-: Sort read 2 aligned file by readname completed.
(-: /home/Juicer_test_run/splits/HC-1.fastq.gz.sam created successfully.
awk: fatal: can't open source file '/opt/juicer/scripts/common/chimeric_blacklist.awk' for reading (No such file or directory)
(-: Sort read 1 aligned file by readname completed.
(-: Sort read 2 aligned file by readname completed.
(-: /home/Juicer_test_run/splits/HC-1.fastq.gz.sam created successfully.
awk: fatal: can't open source file '/opt/juicer/scripts/common/chimeric_blacklist.awk' for reading (No such file or directory)
***!Failure during chimeric handling of /home/ruchi/splits/HiC-1.fastq.gz.
Kindly help.
Neva Durand
Dec 1, 2019, 6:51:02 AM12/1/19
to Ruchi Choudhary, 3D Genomics
Check your installation. Did you follow the steps in the quick start? What do your files and directories look like?
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Neva Cherniavsky Durand, Ph.D.
Assistant Professor, Aiden Lab
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