"Failure during chimera handling of" error on CPU version

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G C

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Jan 3, 2021, 2:58:18 PM1/3/21
to 3D Genomics
Dear all,

I am stuck with Juicer pipeline obtaining the error "Failure during chimera handling of " and I find it difficult to solve the issue.

I am running the CPU code on a MacBookPro 2019 OS Catalina. The Juicer code is up to date (03/01/2021).

Thanks a lot

Neva Durand

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Jan 3, 2021, 3:36:57 PM1/3/21
to G C, 3D Genomics
Is that the only error message you see? Can you post more information?

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Neva Cherniavsky Durand, Ph.D. | she, her, hers
Assistant Professor |  Molecular and Human Genetics
Aiden Lab | Baylor College of Medicine

giaco...@gmail.com

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Jan 3, 2021, 3:45:38 PM1/3/21
to 3D Genomics
This is the full error message.
[main] Version: 0.7.17-r1188
[main] CMD: bwa mem -SP5M -t 8 /Users/giacomocorleone/Tools/Juicer/juicer/scripts/references/Homo_sapiens_assembly19.fasta /Users/giacomocorleone/Tools/Juicer/juicer/scripts/splits/IRE-LalB-CTR-001-HIC-CS-080720_R1.fastq.gz /Users/giacomocorleone/Tools/Juicer/juicer/scripts/splits/IRE-LalB-CTR-001-HIC-CS-080720_R2.fastq.gz
[main] Real time: 939.772 sec; CPU: 6537.997 sec
(-:  Align of /Users/giacomocorleone/Tools/Juicer/juicer/scripts/splits/IRE-LalB-CTR-001-HIC-CS-080720.fastq.gz.sam done successfully
awk: calling undefined function and
 input record number 89, file /Users/giacomocorleone/Tools/Juicer/juicer/scripts/splits/IRE-LalB-CTR-001-HIC-CS-080720.fastq.gz.sam
 source line number 298
***! Failure during chimera handling of /Users/giacomocorleone/Tools/Juicer/juicer/scripts/splits/IRE-LalB-CTR-001-HIC-CS-080720.fastq.gz

Thanks

Neva Durand

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Jan 3, 2021, 5:04:30 PM1/3/21
to giaco...@gmail.com, 3D Genomics
You need to have an updated version of awk / gawk installed on your system (it's not understanding "and")


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