Effects of Solvents on Fluorescence of Biological Dyes

[David haffner sr]

5 Biological Dyes in Water and Isopropyl Alcohol, The purpose of this study was because I began to notice that some of my samples would not fluoresce under certain conditions i.e., Rhodamine B in ethanol will fluoresce brightly in a dilute solution of only 100ppm or 100ppb, where in this study (solvent used was 90% distilled water in 10% Isopropyl alcohol,) I still used 2 drops of rhodamine b, but I had to use the stock solution of 20% to get fluorescence. Carbol fuchsin did not fluoresce because of its weak interaction with this particular buffer solution but does have a recorded emission of 630nm. Fast green has weak fluorescence because it is a triarylmethane, which is similar in part to Malachite green, an arylmethine dye. So samples 2, 4 and 5 are included with their respective emission plots, with samples 1 and 3 included in the absorption data.

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Dave H