Mitochondrial morphology imaging

[Rayees Padder]

Hello,

i am a PhD student and i have to view mitochondrial dynamics in cells, in vitro. can any body tell me that shall i use only mitotracker red or any other dye either in combination with mito red or alone. Or can i use antibodies against cytochrome c and the secondary antibody tagged with red fluorophore? 

i am confused, which option is the best and also if i wish to observe mitochondrial morphology in resected tisse, will the protocol be same or?  

[krishna kumar ganta]

i don know if its very late to reply. mitochondria morphology can be viewed with mitotracker red dye using a good confocal microscopy. all u need to remember is the dye is membrane potential dependent . if u have a mtRFP plasmid u can make a stable cell line and then do your experiments on it.and this method is membrane potential independent .

[Sg A]

Hello,

Sorry for my late response, maybe it will be helpful for some beginners in the field.

Please be advised, that Mitotrackers are supposed to be used in living cells, not in fixed ones.

Info on how to observe mitochondrial morphology is available at https://www.thermofisher.com/ru/ru/home/life-science/cell-analysis/cell-structure/mitochondria.html .

(My response is impressed by Scigine (http://www.scigine.com/)