$400 for 200uL YOYO-1 DNA dye, is there cheaper bis-intercalator dye?

[Nathan McCorkle]

I came across this, it was used to visualise single DNA molecules in nanochannels... I'm guessing it's good at this because it has two dye molecules just nanometers apart, which also have high affinity to DNA and 1000X fluorescence intensity increase upon binding.

It's crazy to me to pay so much though, Its been around since mid-90s at least...

http://products.invitrogen.com/ivgn/product/Y3601

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-Nathan

[xmort]

good for you, the link shows me country specific pricing and its $850:}

I am not sure this has been around for so long. . This is really specialised dye and  in these kind of applications those who need it wil pay for it from their deep pockets. For the rest of us -we have to look for substitutes   and work arounds. Or do some more main stream science.

tm

 

Dne úterý, 20. listopadu 2012 23:20:19 UTC+1 Nathan McCorkle napsal(a):

[Nathan McCorkle]

Wow, well google scholar is showing the earliest relevant (DNA/cell dye) results for yoyo-1 being in 1993, so it's at least 19 years old.

Luckily I think I'll only need a small amount of this stuff, if I even need it at all. Too bad no one here knew of cheaper bis-intercalator alternatives :/

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-Nathan

[ewaldte]

Hi,

SETA BioMedicals has the same product for a much lower price. http://www.setabiomedicals.com/k8-2100.html

Jovita

[John Ladasky]

It has been ages since I worked with the bis-intercalating DNA dyes, but... as I recall, they are shipped in reasonably high concentrations in DMSO solvent, and you dilute them quite a lot to make aqueous working solutions.  They have extremely low Kd values, on the order of 10 nM.  So one vial should last you a very long time.

[ukitel]

One vial would last you forever, maybe you can ask for a sample: chances are that it would be way more that what you need for your experiments
Other DNA intercalating dyes: propidium iodide, DAPI, Hoechst