Could I harvest bacteria and fungi for cleaning agents at home?

[Ulysses1994XF04]

Just an idea I've been toying around with in my head for a while. Do you think it would be possible to harvest bacteria and fungi that I grow on things like food waste, shredded newspapers, grass clippings, etc and harvest them for cleaning agents to use around the house (enzymes, membrane lipids to make soap, etc)?

If so, what would I need to do that? What type of tools, equipment and consumer-accessible reagents would I need (media, buffers, etc)?

[John Griessen]

On 11/04/2012 07:35 PM, Ulysses1994XF04 wrote:
> Do you think it would be possible to harvest bacteria and fungi that I grow

on things like food waste, shredded newspapers, grass
> clippings, etc and harvest them for cleaning agents to use around the

house (enzymes, membrane lipids to make soap, etc)?

The time honored way is to use your compost to sun grow oil stocks like
olive trees, soybeans, castor beans or cotton if dry near the desert, etc.

Do you want to synthesize that process? If you're looking at practical
results, maybe optimize a plant "oil stock" that already does that
process, but maybe microbes can do it better -- not from
ordinary dirt and sun farming? The first question in my mind is, where does the
energy come from without photosynthesis?

http://www.igidr.ac.in/pdf/publication/1306149211s_PP-051.pdf says,
"Although India ranks among the largest producers of oilseeds in the world such as USA, China and Brazil, its productivity is
quite low." [circa 2001]

Will India become a big veg oil exporter, or do you have an idea for growing oil locally
by efficient spending? What does that look like? Vats? Fungus heaps? Fields? Caves?

[Ulysses1994XF04]

That seems like a pretty good idea! But what about during fall and winter? I was thinking bacteria and fungus because I could just grow it indoors all year round; perhaps if I built some indoor hydroponic system, I could plants indoors as well. What are some good, small oily plants?

Also, how would you effectively harvest their oils and lipids? I was thinking maybe column chromatography? Maybe buy a buret online and some silica gel, pack it and run it through to separate oils from the cell components? Would that work? What kind of mobile phase would I need?

[Nathan McCorkle]

There are some commercial products like that, but instead of culturing the bacteria/fungi for cleaning agents, you simply add the organisms to the surface/substrate you want to clean.

Nature's Miracle pet/carpet deodorizer spray has 'bacillus spore blend' as an ingredient:

http://www.petco.com/product/113477/Natures-Miracle-Ultra-Deodorizing-Odor-Control-Formula-Dog-Spray.aspx

Question: What is the Bacillus Spore Blend?

Bacillus Spore Blend is listed in the ingredients for the Ultra-Grooming Spray Conditioning Formula. Some bacillus can be harmful. What is this?

Answer:

Thank you for contacting United Pet Group. This product contains a “bio-enzymatic odor locking” formulas with beneficial microorganisms specifically selected for the production of a range of enzymes to support removal of odors. The Bacillus strains in our products are not harmful at all, even at higher amounts than present in our products. Therefore this product is perfectly safe to use.

Question: Are there any safety issues?

My sister was visiting with her sheltie who has leukemia and has had chemo. quite recently She wanted to use these products since I was testing them with good results on my 5 dogs, but was concerned when she read the product ingredients list. Bacillus? Any problem with dogs using this who have serious immune problems?

Answer:

Thank you for your interest in our product. It is best not to use this product on an immuno-compromised pet. The Bacillus spore blend is used to release enzymes when in contact with odors or soil. This is generally a safe process but would not be recommened in this case. The dog having Leukemia and recent chemo would be more sensitive that a dog with a normal immune system. Check with the dog's vet for cleaning suggestions.

there are more pro looking google results for that quote as well

http://www.midwestcleaningtech.com/scoc.asp
Esteam Pet Stain & Odor Remover - 
A blend of odor neutralizing agents and stain releasing compounds. Pet Stain & Odor Remover chemically neutralizes the odor utilizing a multi-Bacillus spore enzyme to digest the source of the odor. pH: 6.7 Dilution Ratio: Ready to use, up to 1:4

Esteam Triple Action - 
With encapsulating properties will eliminate odor problems. Using advanced technology, Triple Action chemically neutralizes odors. It is extremely effective on a broad spectrum of odors including urine, vomit, skunk and many other malodors. This product efficiently removes odors associated with human, animal, food and smoke. Utilizing a multi-Bacillus spore enzyme, Triple Action eliminates the odor, digests the source and leaves behind a pleasant fragrance. Diluted pH: 6.7 Dilution Ratio: Up to 1:4


and this 

http://www.atomesbio.com/pdf/bio-culture.pdf

ctrl-f bacter here:

http://www.atomesbio.com/products.php

If those products cover what you want, maybe you can clone them by just adding a bit to some broth? Or maybe you want to clean different stuff?

On Sun, Nov 4, 2012 at 5:35 PM, Ulysses1994XF04 <ben...@comcast.net> wrote:

Just an idea I've been toying around with in my head for a while. Do you think it would be possible to harvest bacteria and fungi that I grow on things like food waste, shredded newspapers, grass clippings, etc and harvest them for cleaning agents to use around the house (enzymes, membrane lipids to make soap, etc)?

If so, what would I need to do that? What type of tools, equipment and consumer-accessible reagents would I need (media, buffers, etc)?

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[Ulysses1994XF04]

Thanks, but the point is, I don't want to have to keep buying soaps or cleaning agents; I'd like to be able to make them at home.

[Matt Lawes]

Check out subtilisin producing strains of Bacillus. That could be the answer.

>matt

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[Iván Esteban Araya]

I can add that in general, the step of extraction and purification of any desiable product in a bioprocess is the more dificult and expensive step. 

If you are trying with bacteria/fungi do not think the organism on nature can produce a high yield, normally genetic transformation is needed. 

And based on my experence:

Lipids: posible

Carbohidrates: easy

Proteins: dificult

Enzimes and other side metabolities: really hard -.-, a expensive equipment could be needed.

 

2012/11/4 Nathan McCorkle <nmz...@gmail.com>

[Nathan McCorkle]

On Sun, Nov 4, 2012 at 6:51 PM, Ulysses1994XF04 <ben...@comcast.net> wrote:

Thanks, but the point is, I don't want to have to keep buying soaps or cleaning agents; I'd like to be able to make them at home.

Did you miss the last line where I said 'If those products cover what you want, maybe you can clone them by just adding a bit to some broth? Or maybe you want to clean different stuff?' 

What I meant was that you could try using those products to jump-start your project, if these companies have a blend of bacteria that are good at cleaning, why not try starting there. $8 for a bunch of spores is cheap.

Wait, you've already asked this question before:

https://groups.google.com/forum/?fromgroups=#!topic/diybio/zbCBac0gllE

[Matthias Bock]

I would be very interested in this chromatography column configuration. I am currently trying to separate and quantify the fatty acid composition of different plant oils.
Please keep me/us posted! :-)

Cheers! Matthias

[Cathal Garvey]

Problem: most cellular lipids are, AFAIK, *phospho*lipids. That is, one
end is conjugated to a highly charged phosphate group, which allows the
lipids to form soluble micelles instead of clumping into droplets of
insoluble oil that can be separated by burette.

To extract "oil" from cellular paste, you might be able to find an
enzyme that cleaves this phosphate group from the phospholipids, leading
to a mix of insoluble fatty acids and free phosphate; this would give
you oil for your saponification (soap) and free phosphate as a
fertiliser, although I wouldn't recommend simply adding it to the soil
as it would be far too strong and prone to runoff. Feeding the
phosphate-rich byproduce to aero/hydro-ponic plants, or simply letting
it get digested into more complex cellular matter by bacteria or fungi
before use as compost, might be wiser.

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[Cathal Garvey]

Wild B.subtilis produces surfactin in acceptable amounts. It's a
peptide-based surfacant, though I imagine it's a bit harsh for use on
skin or clothes. Other related strains (which should probably be called
B.subtilis if it weren't for what appears to be a "gold-rush" of species
naming rights) which also occur globally are known to produce more or
less surfactin and other byproducts.

B.subtilis in stationary phase also produces lots of proteases, which
might be helpful in digesting away some stains.. but would probably ruin
silk! :P

On 06/11/12 07:52, Xabier V�zquez Campos wrote:
> You can use lipid accumulating microorganisms such *Lipomyces starkeyi *and
> *Cryptococcus curvatus *which can accumulate up to 40-70% of dry weight as
> lipids and make soap. More info in this document<http://www-06.all-portland.net/bst/030/1047/0301047.pdf>
> .
>
> Or, you can try to isolate biosurfactant-producing microorganisms and use
> their surfactants. Many commercial detergents include surfactants of
> microbial origin. The problem would be isolate the microorganism of
> interest (and non-pathogenic). Probably, starting with a minimal medium
> adding diesel as sole carbon source, 1-2 weeks incubation, isolation in
> rich medium and test surfactant properties of isolates in pure cultures.
> If you know any place affected by oil, diesel, gasoline,... spill or a soil
> close to a petrol station, are good source samples to get faster and higher
> output.
>
> El martes, 6 de noviembre de 2012 01:47:40 UTC+11, Cathal escribi�: