Cleaning and preparing tips

[Meow-Ludo]

Do I need to treat my micropippet tips with DNAse for use in PCR? Or should autoclaving them be enough for them to be sterile?

[Nathan McCorkle]

They should come from the factory certified to be DNase (and possibly
RNase) free, otherwise I'm not sure how to remove DNase other than
rinsing with a chaotropic salt solution or phenol:chloroform rinsing
(but then you'd have to rinse with alcohol after)... for RNase it's
generally recommended to use DEPC to clean them up

http://bitesizebio.com/articles/rnase-and-depc-dispelling-the-myths/

On Wed, Jul 18, 2012 at 2:41 AM, Meow-Ludo <stuart....@gmail.com> wrote:
> Do I need to treat my micropippet tips with DNAse for use in PCR? Or should
> autoclaving them be enough for them to be sterile?
>

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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[Meow-Ludo]

Thanks for that Nathan. I got my tips in a little sandwich bag as a 'gift' for purchasing two pippetors. I think I might stick them in the autoclave and keep an eye out for contamination.

Meow