Creating synthetic organelles

[Koeng]

A few days ago I had an idea when I was searching about chloroplasts (and how people use the PEG i have to put them into plants :). Here it is-

Would it be possible to insert modified E coli that exported the protein for resistance to chloramphenicol, therefore making the E coli like a big BAC? Or would this take too much energy from the yeast? (Currently I don't work with yeast)

Once inside I also had the idea for a yeast plasmid to hold bacteriophage polymerase (for creating it) and then would export the bacteriophage RNA polymerase to nearby E coli cells which would intake the polymerase, and begin expressing a certain gene, or completing a task that could perhaps escaping the cell (any ideas here?) and releasing a virus to target yeast while another bacteria on the inside of the cell, that was triggered from the SAME polymerase (kind of like the lambda lytic and lysogenic operons) that conferred to resistance to that virus by perhaps... (any ideas here?). Although this is one example, I think there are many things that the "worker" cells could do for the yeast.

Anyone have any input? 

Koeng

(also please forgive anything major I forgot or the spelling, I wrote this off the top of my head)

[Mega]

Hi, sounds good...

 

But:

The plant must have some kind of an immune system, because else they would constantly invaded by bacteria of all kinds.... So you would select for the plants with the weakest resistance against bacteria. Those who can kill the bacteria (good trait) are dying from chloramphenicol, while the *immune surpressed* survivie because they keep the bacteria. In free nature, other bacteria will invade and kill those plants then...

[Nathan McCorkle]

On Mon, Mar 4, 2013 at 5:19 PM, Koeng <koen...@gmail.com> wrote:
> A few days ago I had an idea when I was searching about chloroplasts (and
> how people use the PEG i have to put them into plants :). Here it is-
>
> Would it be possible to insert modified E coli that exported the protein for
> resistance to chloramphenicol, therefore making the E coli like a big BAC?

I think the problem with that approach is that the yeast would still
be relatively unconnected to the e.coli, or connected in the wrong
way. As Mega pointed out, the plant might see the e.coli inside and
start attacking, or simple apoptose itself. The e.coli would simply be
e.coli inside a yeast cell, so I think there would be a lot more work
to get it hooked up as a sub-yeast worker.

Another problem might be that the photosynthetic leaf cells probably
would grow more slowly since the photosystems are being blocked
partially by non-photosynthetic e.coli... so maybe you could find a
way to only add the e.coli worker to non-photosynthetic cells?


Why not start with the plastid instead, and look at modifying a
version of it. It would be really cool to have a custom organelle to
do chemistry in, away from the other cell contents!



--
-Nathan

[Nathan McCorkle]

Found this, looks open-access:

Internalization of bioluminescent Escherichia coli and Salmonella
Montevideo in growing bean sprouts

K. Warriner, S. Spaniolas, M. Dickinson, C. Wrightand W.M. Waites
Divisions of Food Sciences, Plant Science, and Agriculture and
Horticulture, School of Biosciences, University of Nottingham,
Leicestershire, UK
2003/0154: received 25 February 2003, revised 23 April 2003 and
accepted 4 May 2003
http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2672.2003.02037.x/full

--
-Nathan