Re: Needing some things

[Mega]

http://www.mobio.com/samples/5
http://www.mobio.com/samples/lb-broth-lennox-powder-growth-media-sample.html

Does that look nice? ;)


Am Samstag, 21. Juli 2012 05:23:01 UTC+2 schrieb SulfurApothecary:

So I was wondering where I can get some LB broth, and e coli cultures. I need it for testing extracts for anti microbial purposes. I was also wondering if anyone has any experiments or such that I could do to start out...

[Dakota Hamill]

So many free things!

[Mega]

Do you already have E.Coli?

[SulfurApothecary]

Thanks the problem with the site above is that it only ships to commercial addresses. I do not have E.Coli but I plan on it.

[Andreas Sturm]

Maybe I can try to send you a lab strain from our university....

But I fear, without them frozen they won't survive until the letter'd have arrived... Anyway, is it legal to send (genetically unmodified) Colis via mail?? 

[Andreas Sturm]

And the ''no delivery to residental adresses'' is *often* not meant so seariously... I'd just  try it  ;) 
Don't mention you're doing diy bio, just write it as you were a lab and  would every day try out samples from companies.


Worst case: they don't answer your mail or  even worse, some conservative may tell you you shouldn't do experiments at home (one of the most stupid attitudes ever!). So you just don't answer back and never mind :)
 

[Cathal Garvey]

For *lab strain* E.coli, my understanding is that they're generally
unregulated unless they're GMO.

At least in Ireland for example, there is a legal framework regulating
Class 2+ bacteria for health and safety reasons. E.coli is in the list,
but with a footnote stating that domesticated lab strains are exempt.

To send E.coli in the post, send a "stab": create a little test tube of
slanted agar, then stab an inoculating loop of E.coli into the agar. You
can cap the tube (E.coli are facultative anaerobes, they won't need air)
and send it in a padded envelope. For added surety, alcohol-spray a
thick plastic ziploc bag, let it dry, put the tube in that before
mailing though. That way, if the tube breaks, the fragments will
possibly still be in a sanitised environment for recovery on the other end.

Label the letter "Non-hazardous biological sample": it's good manners
and could avoid trouble if customs at either end take exception to test
tubes of unidentified bacteria. Ideally, include a little letter slip in
the envelope describing the strain in case customs decide it's worth
opening. I've never heard of one being opened though; labelling it is
pretty "transparent". Postal service workers in most countries haven't
caught the "OMG BIOTERRORISTS" bug yet, it seems. :)

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