Free-NA*

[Avery louie]

In the interest of promoting DIYBIO, I have an offer to make to those of you who have yet to set up a lab/do hands on stuff.

I want to give you FREE PLASMIDS/consumables for producing GFP in e coli.  I will even ship you most of the buffers you need.  This will cut down costs on things that you only need a tiny amount of, and would otherwise make this procedure expensive.  The caveats are:

- It will come dry, on paper,  You will need to elute it with TE, which will come with the DNA.  I haven't used this method before, but the literature (any many people) report that it will work.
- You have to send me photos of your results and procedure.  I am going to trust that you will do this.
- You have to send me a receipt/picture of the rest of the stuff you need to do the experiment (listed below) before I ship
- I will only ship 5 of these packages

If you are interested, email me back with pictures/a receipt showing you bought the stuff, and your address/name/shipping info.

What you get:
50 mM CaCl2, enough for 2 transformations
Some TE for to elute the pDNA off the paper
A piece of paper with the pDNA on it
LB broth, enough for 2 transformations
Instructions on how to do the transformation

What I need pictures of:
your ice bath (some kind of cup you can fill with ice)
your hot water bath (water with some way of showing it is 42 C, think thermometers)
some source for e. coli (Carolina sells these for like, $10)
petri dishes (another Carolina item)
LB agar media
ampicillin antibiotic (Carolina, goldbio)
sterile pippettors of some kind
sterile loops or a wire loop (and a way to sterilise your loop, like fire)
a microwave or stove for melting the agar

The price of this stuff from Carolina is ~$50:

http://www.carolina.com/nav/i/category/living+organisms/prokaryotes/bacteria+cultures+and+sets/mm294+slant+culture/r/price+range/under+%2425/n/214.do?sortby=ourPicks

http://www.carolina.com/product/living+organisms/biological+media/biological+media/luria+broth+agar+ready-to-pour+media+set.do?sortby=ourPicks (lb + ampicillin)

http://www.carolina.com/product/nichrome+wire+inoculating+loop.do?keyword=loop&sortby=bestMatches

http://www.carolina.com/product/disposable+plastic+needle-point+pipets.do?keyword=transfer+pippete&sortby=bestMatches

--Avery

[Avery louie]

The water bath only has to be at 42 c for 90 seconds.  In most cases, microwaved water works fine (you just have to get it to 42 C)

--A

[Jonathan Cline]

On Sunday, June 3, 2012 4:45:37 PM UTC-7, Avery wrote:

I want to give you FREE PLASMIDS/consumables for producing GFP in e coli. 

Excellent offer.  e coli is smelly however.  What else ya got?

-- 
## Jonathan Cline
## jcl...@ieee.org
## Mobile: +1-805-617-0223
########################

 

[Mega]

It probably wont work with fungi, I'm sorry. But the bacterial promotor and terminator won't be recognized by fungi. maybe you get some background expression too. The plasmid wont replicate in fungi also.

[Jonathan Cline]

On Wednesday, August 29, 2012 4:51:28 AM UTC-7, Mega wrote:

It probably wont work with fungi, I'm sorry. But the bacterial promotor and terminator won't be recognized by fungi. maybe you get some background expression too.  The plasmid wont replicate in fungi also.

A halobacteria (Halobacterium sp. NRC-1) kit offering would be quite nice, pretty, and harmless:

https://groups.google.com/d/topic/diybio/icNC21eVJ7M/discussion

...To make the little pinks glow green... but if these guys have positive phototaxis, would that make them narcissistic when they glow?    Hrmmm...