Polycistronic expression

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Mega

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Mar 9, 2013, 12:50:14 PM3/9/13
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Hi everyone, 

Bacteria and plastids are capable of having many coding sites per mRNA molecule. 

that wold give us a mRNA of       5' cap - RBS - CDS1 - spacer - CDS 2 - spacer - CDS3 - 3'UTR

Now I read the rumor that each CDS needs its own ribosome binding site (SD-sequence)... True? 

On the other hand there is this paper saying that for some chloroplast genes, no RBS are needed for expression neither in chloroplasts nor in E.Coli ..


So what is true when linking two genes? Include another RBS or not?? 


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