cell dissociation papain

[biowarrior]

hi i got a question. I would like to dissociate papain a protease enzyme from a powdered form. i know i have to dissolve it first but how would i buffer it to a PH that its active.. i'm using this for a cell culture experiment but i want to make sure i dissolve the papain correctly that its active

can anyone give me any tips or protocols that are simple

thanks

perry

[Dakota Hamill]

http://www.worthington-biochem.com/pap/default.html 

That website says it's pretty thermostable (up to 60 to 70C )and most active from 6-7 pH

http://en.wikipedia.org/wiki/Papain also states its use in cell culture to digest extra-cellular matrix in just 5-10 minutes, and gives the citation [7]

Sounds like it's as simple as "just add water", although for a starting concentration I'd have no idea.

[Dakota Hamill]

that's a nifty enzyme website, never seen it before.  just as I sent the previous email I found this on the right side

http://www.worthington-biochem.com/pap/assay.html

has some info on it's activity