Anyone interested in trading for 2-Mercaptoethanol? I am in need of other lysis buffer ingredients.

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Conner Berthold

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Mar 30, 2013, 8:23:47 PM3/30/13
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Kinda a weird post but I bought 25mL of 2-Mercaptoethanol from Sigma (M3148-25ML Suitable for Cell culture, Electrophoresis...) but no longer need it for my protocols. I am however in need of some of the below reagents.
  • NP-40 or Triton X-100
  • sodium deoxycholate
  • Anything else? Just add a message!

Let me know if your interested! I would prefer that this was to be used in a lab (with a fume-hood...) as it is not the best chemical to deal with.


-Conner
cbertholdbio at gmail dot com

Mega

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Apr 1, 2013, 3:34:58 PM4/1/13
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Could possibly be that we have Triton X-100, but have never used it. 

Will look it up tomorrow. 


How much would you need? 

Conner Berthold

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Apr 1, 2013, 4:14:45 PM4/1/13
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Thanks!
It is for RIPA buffer for Cell lysis, at 1%, so a few grams (10g max) would be plenty.

-Conner

Mega

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Apr 9, 2013, 9:50:02 AM4/9/13
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Sorry for the late reply, but didn't find any... :(
 
The only thing we have that sounds similar is Trizma...

Mega

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Jun 25, 2013, 3:07:07 PM6/25/13
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Hi Conner,

I was able to get some milliliters TritionX-100... If you still need it I'm gonna send you.
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