Re: [DIYbio] Where to buy PCR primers/oligonucleotides?

[John Griessen]

On 06/30/2012 01:35 PM, jarlemag wrote:
> I've looked up quite a few suppliers, but all of them require listing which organization you belong to for registering and/or
> shipping.

So, you create a company identity for yourself. File a doing business as fictitious name in your county.
Pay for the order with a check from the dba account with the fictitious name. That's all you need... well,
bookkeeping and a few other things maybe...and taxation, of course.

[Cathal Garvey]

Better if we can find companies that don't discriminate and give them our custom.

I ordered my plasmid to be synthesised from Epoch Life Sciences, I'd say they do oligos too. They were reasonable and friendly, too. Payment by credit card.

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[Nathan McCorkle]

If it's for iGEM, why not list your school as the institution???

On Sat, Jun 30, 2012 at 2:35 PM, jarlemag <jarle...@gmail.com> wrote:
> Hi. So, I just got my OpenPCR kit, which I must admit I bought somewhat on a
> whim. I'm a Msc. student in biotechnology, and like the idea of doing some
> "research" on my spare time. I'm participating in our university's iGEM
> team, and for the last couple of weeks we've been working full-time on our
> project and getting more lab experience. We haven't gotten to running PCR
> yet, but that's coming up. It would be fun to put my own machine to use too
> (if I can manage to assemble it). I'm having one problem though - where can
> I get primers synthesized and the other reagents I need (I don't want to
> take anything from the lab, and would like to be able to design and buy my
> own primers separate from our project)?

>
> I've looked up quite a few suppliers, but all of them require listing which
> organization you belong to for registering and/or shipping.
>

> The suppliers I have checked out include:
>
> eurofins mwg|operon
> IDT DNA
> Invitrogen/Life Technologies
> Sigma Aldrich
> VWR
> GenScript
> BioLegio
>
>
> Does anyone know of any suppliers that ship to residential addresses, and
> don't require an institutional affiliation?
>
> Thanks for your attention,
>
> Jarle
>
> PS: I'm located in northern Europe

>
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

[Dakota Hamill]

My best friend from school did oligo synthesis for a company and we almost got their old DNA synthesizer, with the hope of producing DIY primers, but the company wanted their old machine back (so people had to buy their new ones, I assume).  Nevertheless, in the next week I may be able to give away some particular barcoding primers that my friend and I are trying to turn into something.  My friend ended up making about 8,000 rxns worth, so, plenty to share.  

I dont know how many oligo's you'd have to sell to break even on a DNA synthesizer, but it'd be cool to look into supplying primers to the "common person".

-Dakota

[Bryan Bishop]

On Sun, Jul 1, 2012 at 6:22 PM, Dakota Hamill <dko...@gmail.com> wrote:

I dont know how many oligo's you'd have to sell to break even on a DNA synthesizer, but it'd be cool to look into supplying primers to the "common person"

It depends on what sort of synthesizer you buy. It looks like the cheapest ones that you can buy that may or may not work start around $15,000. The $1,000 items listed on ebay don't work. But if you were to buy some refurbished or new synthesizer, you're looking closer to $50,000. So, that's a lot of primers - at least a few thousand.

- Bryan
http://heybryan.org/
1 512 203 0507

[Ashley Heath]

Shoot me an email to ahe...@sial.com

[Josh Perfetto]

Also just because they ask for a company doesn't mean that they won't
ship to individuals. Try putting "none" if the webform requires it and
see what happens when you place an order.

-Josh

[jarlemag]

I looked at their website, but it didn't say anything about oligo synthesis.

kl. 11:34:04 UTC+2 søndag 1. juli 2012 skrev Cathal følgende:

Better if we can find companies that don't discriminate and give them our custom.

I ordered my plasmid to be synthesised from Epoch Life Sciences, I'd say they do oligos too. They were reasonable and friendly, too. Payment by credit card.

John Griessen <jo...@industromatic.com> wrote:

>On 06/30/2012 01:35 PM, jarlemag wrote:
>> I've looked up quite a few suppliers, but all of them require listing
>which organization you belong to for registering and/or
>> shipping.
>
>So, you create a company identity for yourself.  File a doing business
>as fictitious name in your county.
>Pay for the order with a check from the dba account with the fictitious
>name.  That's all you need... well,
>bookkeeping and a few other things maybe...and taxation, of course.
>
>--
>You received this message because you are subscribed to the Google
>Groups "DIYbio" group.
>To post to this group, send email to diy...@googlegroups.com.
>To unsubscribe from this group, send email to

>diybio+unsubscribe@googlegroups.com.

[jarlemag]

I already did when registering for one site, but I really don't want the department officials to come asking if the company does any verification or will ship to the department adress. (I guess it probably wouldn't do any big harm, but I'm a rather careful person).

> diybio+unsubscribe@googlegroups.com.

[jarlemag]

I tried that with one site, but shortly thereafter got an email with "sorry, we don't give accounts to individuals". Guess I might try it with more companies, but I'm quite shy and would prefer to go under the radar where possible.

kl. 18:59:10 UTC+2 søndag 1. juli 2012 skrev Josh Perfetto følgende:

Also just because they ask for a company doesn't mean that they won't
ship to individuals. Try putting "none" if the webform requires it and
see what happens when you place an order.

-Josh


On Sun, Jul 1, 2012 at 2:34 AM, Cathal Garvey <cathal...@gmail.com> wrote:
> Better if we can find companies that don't discriminate and give them our custom.
>
> I ordered my plasmid to be synthesised from Epoch Life Sciences, I'd say they do oligos too. They were reasonable and friendly, too. Payment by credit card.
>
> John Griessen <jo...@industromatic.com> wrote:
>
>>On 06/30/2012 01:35 PM, jarlemag wrote:
>>> I've looked up quite a few suppliers, but all of them require listing
>>which organization you belong to for registering and/or
>>> shipping.
>>
>>So, you create a company identity for yourself.  File a doing business
>>as fictitious name in your county.
>>Pay for the order with a check from the dba account with the fictitious
>>name.  That's all you need... well,
>>bookkeeping and a few other things maybe...and taxation, of course.
>>
>>--
>>You received this message because you are subscribed to the Google
>>Groups "DIYbio" group.
>>To post to this group, send email to diy...@googlegroups.com.
>>To unsubscribe from this group, send email to

>>diybio+unsubscribe@googlegroups.com.

>>For more options, visit this group at
>>http://groups.google.com/group/diybio?hl=en.
>
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> Sent from K-9 Mail on Android
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[Avery louie]

I used IDT.  No problem except that I wasn't home when my primers arrived >.<

--A

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