Expressing pVIB in Cyanobacteria

[Chowe]

Hello, I was just thinking of different possibilities to express pVIB after having expressed it in E.Coli. I see that both cyanobacteria and E.coli are both gram-negative bacteria. Has anyone here tried expressing pVIB in cyanobacteria? I am not very knowledgable about cyanobacteria, so if it is possible, how long would the culture last since it gets its energy from the sun? My goal is try to create a Bio-lamp of some sorts, so I thought this would be interesting to try. Any ideas or information would be helpful. Thanks!

Corey 

[Sebastian S. Cocioba]

Maybe this is a viable lead. Its fairly recent circa 2001. http://onlinelibrary.wiley.com/store/10.1111/j.1574-6968.2002.tb11012.x/asset/j.1574-6968.2002.tb11012.x.pdf;jsessionid=9B6B14AB3A2120E096B3F50194D96A2E.d03t04?v=1&t=hcghpfn9&s=b6071ad58dc638e998ce3971d7c79594abc5f7c0

Sebastian S Cocioba

CEO & Founder

New York Botanics, LLC

Sent via Mobile E-Mail 

On Jan 27, 2013, at 12:48 PM, Chowe <c9h...@Yahoo.com> wrote:

Hello, I was just thinking of different possibilities to express pVIB after having expressed it in E.Coli. I see that both cyanobacteria and E.coli are both gram-negative bacteria. Has anyone here tried expressing pVIB in cyanobacteria? I am not very knowledgable about cyanobacteria, so if it is possible, how long would the culture last since it gets its energy from the sun? My goal is try to create a Bio-lamp of some sorts, so I thought this would be interesting to try. Any ideas or information would be helpful. Thanks!

Corey 

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[Chowe]

Im afraid it says that the link is forbidden so I cannot see it!

[Jeswin]

On Sun, Jan 27, 2013 at 4:45 PM, Chowe <c9h...@yahoo.com> wrote:
> Im afraid it says that the link is forbidden so I cannot see it!
>

same here. Do you have a citation, Sebastian?

[Sebastian S. Cocioba]

Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping

Sorry for that, Ill check all links from now on. 

Sebastian S Cocioba

CEO & Founder

New York Botanics, LLC

Sent via Mobile E-Mail 

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[Patrik D'haeseleer]

There have been several iGEM teams working wit cyanobacteria as well, including one team last year that tried to do  bioluminescence. Not sure how far they got though:

http://2012.igem.org/Team:Copenhagen
http://openwetware.org/wiki/IGEM:Harvard/2006/Cyanobacteria/Protocols
http://2008.igem.org/Team:Hawaii
http://2010.igem.org/Team:Utah_State
http://2011.igem.org/Team:VCU

Patrik

[Mega]

I once asked a proff for that.

He said that the Ori's are well conserved usually, so that the E.Coli origin may work in Cyanobacteria too.

The  promoter may be a weak point, but it may work due to being derived from a gram negative bacterium.