Fwd: 7 days until Primer Day - primers for a penny

[Nathan McCorkle]

---------- Forwarded message ----------
From: Invitrogen <em...@invitrogenemail.com>
Date: Thu, Oct 25, 2012 at 1:31 PM
Subject: 7 days until Primer Day
To: nmz...@gmail.com

1 day only!

On November 1, 2012 place an order for primers and you will only pay a penny for a primer!* Use the promotion code below when placing your order. This promotion is only valid for one day so mark your calendars now! Use promotion code: US – P915999 / CA – P915993 Promotion excludes TaqMan® assays, TaqMan® primers, and TaqMan® probes. Get your free primers in 7 days

FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC PROCEDURES. © 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. In compliance with federal regulations, we hereby disclose that this email communication is for commercial purposes. *Terms and Conditions: This promotion is open to customers in the US and Canada. Promotion excludes Taqman® assays, primers, and probes. Discount will apply to orders received by Life Technologies on November 1, 2012. Discount does not include shipping and handling. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply

--
-Nathan

[Nathan McCorkle]

I wonder if there is a limit... gibson assembly orders?

On Fri, Oct 26, 2012 at 3:09 AM, Tristan Eversole
<custome...@trioptimum.com> wrote:
> Thank you for letting me know about this.
>
> I kind of have a lot of work to do before Nov. 1 now.
>
> --T.
>
> On Oct 25, 2012, at 11:43 PM, Patrik D'haeseleer wrote:

>
>>
>>
>> On Thursday, October 25, 2012 6:56:16 PM UTC-7, Nathan McCorkle wrote:
>>
>>
>> ---------- Forwarded message ----------
>> From: Invitrogen <em...@invitrogenemail.com>
>> Date: Thu, Oct 25, 2012 at 1:31 PM
>> Subject: 7 days until Primer Day
>> To: nmz...@gmail.com
>>
>>
>>

>> 1 day only!
>>
>> On November 1, 2012 place an order for primers and you will only pay a penny for a primer!* Use the promotion code below when placing your order. This promotion is only valid for one day so mark your calendars now!
>>
>> Use promotion code: US – P915999 / CA – P915993
>>
>> Promotion excludes TaqMan® assays, TaqMan® primers, and TaqMan® probes.
>>
>> Get your free primers in 7 days
>>
>> FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC PROCEDURES.
>> © 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. In compliance with federal regulations, we hereby disclose that this email communication is for commercial purposes.
>>
>> *Terms and Conditions:
>> This promotion is open to customers in the US and Canada. Promotion excludes Taqman® assays, primers, and probes. Discount will apply to orders received by Life Technologies on November 1, 2012. Discount does not include shipping and handling. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply
>>
>> --
>> -Nathan

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--
-Nathan

[Brian Degger]

Any know about similer offers for eu?

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--
----------------------------------------
Brian Degger

twitter: @drbrian

http://makerspace.org.uk

http://transitlab.org

----------------------------------------

[Nathan McCorkle]

There was a code for Canada... not sure if they're part of the EU
since they hail to the Queen of England.

-Nathan

[Eric Ma]

We Canadians aren't EU members.

Cheers,
Eric
-----------------------------------------------------------------------
Please consider the environment before printing this e-mail. Do you really need to print it?

http://about.me/ericmjl

[Josiah Zayner]

Still, most primers are less than 30mers. $0.30 is still nothing.

On Tuesday, October 30, 2012 5:04:40 PM UTC-5, Lauri Reinhold wrote:

Check this out!! They completely changed the promotion!!! And after talking to them, they refuse to honor the original promotion!

[Bryan Bishop]

On Tue, Oct 30, 2012 at 5:32 PM, Josiah Zayner <josiah...@gmail.com> wrote:

Still, most primers are less than 30mers. $0.30 is still nothing.

But that's 30x more than they were offering at.

- Bryan
http://heybryan.org/
1 512 203 0507

[Derek]

I'd be interested in what other folks are ordering. I need to do a bit more research on what the most common barcoding primer sets are that I want. I'll post back by tomorrow night if anyone else is interested.

fyi - had to create an account and answer questions from a human, so register tomorrow if you want to order thurs.

Derek

[Tristan Eversole]

So... wait, I can only order 10 oligos (5 primer sets)? Ooooh, tough choice.... :(

On Oct 30, 2012, at 3:04 PM, Lauri Reinhold wrote:

> Check this out!! They completely changed the promotion!!! And after talking to them, they refuse to honor the original promotion!
>
> On Thursday, October 25, 2012 9:56:16 PM UTC-4, Nathan McCorkle wrote:
>
>

> ---------- Forwarded message ----------
> From: Invitrogen <em...@invitrogenemail.com>
> Date: Thu, Oct 25, 2012 at 1:31 PM
> Subject: 7 days until Primer Day
> To: nmz...@gmail.com
>
>
>

> 1 day only!
>
> On November 1, 2012 place an order for primers and you will only pay a penny for a primer!* Use the promotion code below when placing your order. This promotion is only valid for one day so mark your calendars now!
>
> Use promotion code: US – P915999 / CA – P915993
>
> Promotion excludes TaqMan® assays, TaqMan® primers, and TaqMan® probes.
>
> Get your free primers in 7 days
>
> FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC PROCEDURES.
> © 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. In compliance with federal regulations, we hereby disclose that this email communication is for commercial purposes.
>
> *Terms and Conditions:
> This promotion is open to customers in the US and Canada. Promotion excludes Taqman® assays, primers, and probes. Discount will apply to orders received by Life Technologies on November 1, 2012. Discount does not include shipping and handling. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply
>
> --
> -Nathan
>

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> <primer2.jpg>

[Lauri Reinhold]

I was actually able to get 15 to go through and some that were over 30 mer.  Good luck!

[Tristan Eversole]

Well, it went pretty well for me-- I got some degenerate primers, and a few that were over 30bp.

For those are interested in what I got:

I ordered two pairs of microsatellite primers reported for Taraxacum officinale (common dandelion) in (2) and successfully used in multiplex PCR in (1), modified with M13(-21) tails as described in (3). That's the dandelion part; now I just lack corresponding M13(-21) oligos with fluorescent tags, and if I somehow manage to get those, I could maybe do some multiplex PCR on dandelion microsatellites and get a fragment analysis from the University of New Mexico.

On the Triops front, I got the COI primers reported from (4), the degenerate primers used to amplify the extant snippet of caudal from T. longicaudatus (reported in a dissertation), and the adaptor primers used with XhoI in (5). Unfortunately, I discovered that I don't have any way to get DNA out of my T. cancriformis specimen after all, so I'm in a jam, because that's my last non-preserved specimen and it'll take two months to get another cohort of critters. I've got to fix this situation as soon as possible.

There was no way I could possibly have afforded this without the Primer Day offer, and I'm very glad I caught word of it.

--Tristan


1. Falque et al. Development and characterization of microsatellite markers in the sexual-apomictic complex Taraxacum officinale (dandelion). Theoretical And Applied Genetics (1998) vol. 97 (1-2) pp. 283-292

2. Matsumoto et al. Alien Dandelions Displace a Native Related Species Through Interspecific Pollen Transfer. Single-Pollen Genotyping (2011) pp. 83-99

3. Schuelke. An economic method for the fluorescent labeling of PCR fragments. Nature biotechnology (2000) vol. 18 (2) pp. 233-4

4. Vanschoenwinkel et al. Toward a global phylogeny of the "living fossil" crustacean order of the Notostraca. PLoS ONE (2012) vol. 7 (4) pp. e34998

5. Heffer and Pick. Rapid isolation of gene homologs across taxa: Efficient identification and isolation of gene orthologs from non-model organism genomes, a technical report. EvoDevo (2011) vol. 2 (1) pp. 7