DMSO

[Koeng]

where do I get this stuff?

-Koeng

[Avery louie]

You can get it on amazon.

--A

On Jan 27, 2013 2:43 PM, "Koeng" <koen...@gmail.com> wrote:

where do I get this stuff?

-Koeng

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[Sebastian S. Cocioba]

Ebay too. Just wear gloves. As you may know it makes small to medium sized molecules fat soluble aka can go through skin. It would be amazing if insulin were small enough to be applied the dmso route. Alas its huge...sigh.

Sebastian S Cocioba

CEO & Founder

New York Botanics, LLC

Sent via Mobile E-Mail 

[Avery louie]

Also, you generally don't need to sterilize it.  If you do, filter sterilize it, and if you filter sterilize it, make sure your filter is solvent resistant...or your filter will dissolve.

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[Eugen Leitl]

On Sun, Jan 27, 2013 at 11:43:10AM -0800, Koeng wrote:
> where do I get this stuff?

Purity, amount? Alibaba, VWR, Amazon, etc.

[Koeng]

Thanks guys! Finally I can get some...

[Sebastian S. Cocioba]

Any particular project in mind?

Sebastian S Cocioba

CEO & Founder

New York Botanics, LLC

Sent via Mobile E-Mail 

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[Eugen Leitl]

On Sun, Jan 27, 2013 at 03:53:51PM -0500, Sebastian S. Cocioba wrote:
> Any particular project in mind?

I'm not Koeng, but ours is biocryopreservation (by means
of perfusion with a sufficiently high molality of colligative
and noncolligative cryopreservatives and homogenous/heterogenous
ice nucleation inhibitors to allow for vitrification
of macroscopic biological systems at ~-130 to -150 C glass point).

[Nathan McCorkle]

I've seen dmso at most "hippy"/natural foods stores, in the soap/cosmetics aisle.

On Jan 27, 2013 11:43 AM, "Koeng" <koen...@gmail.com> wrote:

where do I get this stuff?

-Koeng

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[Koeng]

X-gal and competent cell preparation 

[Bjonnh]

On Sun, Jan 27, 2013 at 03:01:10PM -0500, Sebastian S. Cocioba wrote:
> Ebay too. Just wear gloves. As you may know it makes small to medium sized
> molecules fat soluble aka can go through skin. It would be amazing if
> insulin were small enough to be applied the dmso route. Alas its
> huge...sigh.
>

Hi,

Be careful, nitrile gloves are not suitable for work with DMSO, use thick rubber ones
(see http://people.ccmr.cornell.edu/~cober/complete.chemical.hygiene.plan.2000.pdf).


--
Jonathan BISSON

E-mail (please use this one from now) : bjo...@bjonnh.net

[Cathal Garvey]

Hey Koeng,
If you're using the PEG/Mg/DMSO method of E.coli transformation, you can
do without DMSO at the cost of an order of magnitude or so in
transformation efficiency.

I've got the protocol without DMSO up here on github:
https://github.com/cathalgarvey/biohacking-protocols

Works quite well using Miralax and generic-brand pharmacy-grade
magnesium sulphate.

On 27/01/13 23:11, Koeng wrote:
> X-gal and competent cell preparation
>
> On Sunday, January 27, 2013 12:53:51 PM UTC-8, Sebastian wrote:
>
> Any particular project in mind?
>
> Sebastian S Cocioba
> CEO & Founder
> New York Botanics, LLC
>
> Sent via Mobile E-Mail
>

> On Jan 27, 2013, at 3:47 PM, Koeng <koen...@gmail.com <javascript:>>

> wrote:
>
>> Thanks guys! Finally I can get some...
>>
>> On Sunday, January 27, 2013 11:43:10 AM UTC-8, Koeng wrote:
>>
>> where do I get this stuff?
>>
>> -Koeng
>>
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>> <https://groups.google.com/d/forum/diybio?hl=en>

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>> <https://groups.google.com/groups/opt_out>.

>>
>>
>
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[Koeng]

Thanks! I am replacing the calcium chloride method I have been using with this one, and I am trying it out tonight! I since my altogether project is to create an open plasmid for halobacterium, I will make this the method-of-choice when I get to making competent halobacterium. To me, halobacterium NCR-1 should be as easy as e coli to use!

-Koeng