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Welcome on the Proteomics Q&A!
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RayS
9/5/23
Quantifying Channel Bleeding in TMT experiments
Is there any quantitative measure/metric to measure the extent of Channel Bleeding in TMT experiments
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Quantifying Channel Bleeding in TMT experiments
Is there any quantitative measure/metric to measure the extent of Channel Bleeding in TMT experiments
9/5/23
Gautam Saxena
8/30/23
Pushing my understanding of TimsTof IonMobility Resolution
For TimsTOF, I *think* that the typical diaPASEF ion mobility separation is for ~100ms. Is this
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Pushing my understanding of TimsTof IonMobility Resolution
For TimsTOF, I *think* that the typical diaPASEF ion mobility separation is for ~100ms. Is this
8/30/23
Maximilian Strauß
, …
david.b...@ipbs.fr
6
1/25/23
How to write mzIdentMLs
Hi Max, If this feature is still useful to you, please open an issue in this repo https://github.com/
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How to write mzIdentMLs
Hi Max, If this feature is still useful to you, please open an issue in this repo https://github.com/
1/25/23
viktori...@fh-hagenberg.at
,
Eric Deutsch
3
5/9/22
Spectrum Library Generation Tool
Thanks Eric, I will have a look at it! Best, Viktoria Eric Deutsch schrieb am Mittwoch, 4. Mai 2022
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Spectrum Library Generation Tool
Thanks Eric, I will have a look at it! Best, Viktoria Eric Deutsch schrieb am Mittwoch, 4. Mai 2022
5/9/22
Dominik Lux
,
david.b...@ipbs.fr
2
12/23/21
Searching for a tool which calculates a MS/MS spectrum metric to tell how well it may be identified
Hi Dominik, I can think about two options but maybe more options exist (in OpenMS?): - there is the
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Searching for a tool which calculates a MS/MS spectrum metric to tell how well it may be identified
Hi Dominik, I can think about two options but maybe more options exist (in OpenMS?): - there is the
12/23/21
dbl...@gmail.com
, …
Jarka Stankova
9
11/17/21
Q&A
convert corrupt thermo raw file
Dear David, Have you been successful with the data recovery? I accidentally delete my thermo raw data
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Mass_Spectrometry
Raw
Thermo
Q&A
convert corrupt thermo raw file
Dear David, Have you been successful with the data recovery? I accidentally delete my thermo raw data
11/17/21
Steven Verbruggen
, …
RayS
4
7/1/21
Mass deviation correction tool?
MS-Fragger too can export calibrated as well as uncalibrated MGF which you can use further. I guess
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Mass deviation correction tool?
MS-Fragger too can export calibrated as well as uncalibrated MGF which you can use further. I guess
7/1/21
viktori...@fh-hagenberg.at
,
johanne...@gmail.com
3
5/7/21
Contaminants Spec Lib
Hi Johannes, great, thanks a lot! Best regards, Viki johanne...@gmail.com schrieb am Freitag, 7. Mai
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Contaminants Spec Lib
Hi Johannes, great, thanks a lot! Best regards, Viki johanne...@gmail.com schrieb am Freitag, 7. Mai
5/7/21
Adriana Domarques
, …
dtabb1973
3
6/1/20
Q&A
Corrupted raw files
Hi, Adriana. I have routinely used this ProteoWizard msConvert line to produce mzML files for later
unread,
Q&A
Corrupted raw files
Hi, Adriana. I have routinely used this ProteoWizard msConvert line to produce mzML files for later
6/1/20
Gautam Saxena
,
Karl Mechtler
2
4/23/20
Q&A
Is it possible to separate peptides/proteins based on abundance?
You can work with monolithic columns from Thermo to seperate peptides and proteins. Am Mittwoch, 22.
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Q&A
Is it possible to separate peptides/proteins based on abundance?
You can work with monolithic columns from Thermo to seperate peptides and proteins. Am Mittwoch, 22.
4/23/20
edit.m...@gmail.com
,
dtabb1973
2
12/13/19
Q&A
proteomic course
In South Africa, most graduate coursework ends at the B.Sc. Honours level. I teach lectures on
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Q&A
proteomic course
In South Africa, most graduate coursework ends at the B.Sc. Honours level. I teach lectures on
12/13/19
Gautam Saxena
, …
David Bouyssié
4
12/2/19
Q&A
removing neutral loss fragments from spectra
Thanks David, I'm going through the paper now.... On Monday, 2 December 2019 01:54:05 UTC-5,
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Q&A
removing neutral loss fragments from spectra
Thanks David, I'm going through the paper now.... On Monday, 2 December 2019 01:54:05 UTC-5,
12/2/19
niki...@gmail.com
, …
Veit Schwämmle
4
7/25/19
Q&A
Isobar tool and file format
Hi Michael, Isobar is not maintained anymore for a longer time and I suggest you to use eg the R
unread,
TMT
isobaric-tags
Q&A
Isobar tool and file format
Hi Michael, Isobar is not maintained anymore for a longer time and I suggest you to use eg the R
7/25/19
Vladimir Gorshkov
5/24/19
Q&A
Multiple injection info on Thermo Q-Exactive
Dear all, does any of you know of any reference information on the scan header information in Thermo
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Instrumentation
Raw
Thermo
Q&A
Multiple injection info on Thermo Q-Exactive
Dear all, does any of you know of any reference information on the scan header information in Thermo
5/24/19
hom...@gmail.com
,
dtabb1973
2
4/27/19
Q&A
Speed-up download from PRIDE
I see that PRIDE supports Aspera protocol. If that's still true, it's likely to be a
unread,
PRIDE
public_data
reanalysis
technical
Q&A
Speed-up download from PRIDE
I see that PRIDE supports Aspera protocol. If that's still true, it's likely to be a
4/27/19
Vladimir Gorshkov
,
Brian Pratt
3
3/29/19
Q&A
tims TOF to mzML conversion
Hi Brian, thank you for your answer. I will "play" with it then. I loooked on the page with
unread,
IMS
data_formats
mzML
Q&A
tims TOF to mzML conversion
Hi Brian, thank you for your answer. I will "play" with it then. I loooked on the page with
3/29/19
Gautam Saxena
1/2/19
Q&A
Understanding dynamic range of QTOF vs Orbi's dynamic range
In Orbitrap instruments, my understanding is that the ~3.5 orders of magnitude instrascan dynamic
unread,
Orbitrap
Sciex
Q&A
Understanding dynamic range of QTOF vs Orbi's dynamic range
In Orbitrap instruments, my understanding is that the ~3.5 orders of magnitude instrascan dynamic
1/2/19
Gautam Saxena
,
David Bouyssié
3
10/15/18
Q&A
is the *order* of nearly isobaric centroided peaks in an orbitrap consistent from scan to scan?
Dear Gautam, It's difficult to answer your question because it totally depends on your full setup
unread,
Instrumentation
Orbitrap
Q&A
is the *order* of nearly isobaric centroided peaks in an orbitrap consistent from scan to scan?
Dear Gautam, It's difficult to answer your question because it totally depends on your full setup
10/15/18
Gautam Saxena
, …
Vladimir Gorshkov
3
9/19/18
Q&A
Is there a way to determine the start/stop LC time when "real" peptides are eluting?
Adding to what David wrote. I can definetly say that there is no obvious signal in Thermo RAW file (
unread,
Fusion
LC
Sciex
Q&A
Is there a way to determine the start/stop LC time when "real" peptides are eluting?
Adding to what David wrote. I can definetly say that there is no obvious signal in Thermo RAW file (
9/19/18
Gautam Saxena
,
David Bouyssié
2
9/19/18
Q&A
Does a peptide's intensity affect elution time?
Hi Gautam, Here is a manuscript we published 2 years ago that should be useful for your research:
unread,
Fusion
LC
RT
quantitation
retention_time
Q&A
Does a peptide's intensity affect elution time?
Hi Gautam, Here is a manuscript we published 2 years ago that should be useful for your research:
9/19/18
Vladimir Gorshkov
,
Timo Sachsenberg
3
8/20/18
Q&A
Averaging of scans in raw file
Hi Timo, thank you for your answer. I will look into it and see if I would like to go this way. Best
unread,
Raw
Q&A
Averaging of scans in raw file
Hi Timo, thank you for your answer. I will look into it and see if I would like to go this way. Best
8/20/18
johanne...@gmail.com
, …
jiangj...@gmail.com
14
6/25/18
Q&A
Isobaric tag analysis with matched pairs
I know this is a very old thread. But I got the same error today using MSstats package to load my
unread,
Bioinformatics
Statistics
isobaric-tags
quantitation
Q&A
Isobaric tag analysis with matched pairs
I know this is a very old thread. But I got the same error today using MSstats package to load my
6/25/18
Gautam Saxena
, …
Brian Pratt
10
4/16/18
batch reading of Sciex wiff files using Sciex dll
Hi Gautam, Good news: there is in fact SWIG support in ProteoWizard, developed for use with Java. Bad
unread,
Linux
Sciex
software_development
vendor_software
batch reading of Sciex wiff files using Sciex dll
Hi Gautam, Good news: there is in fact SWIG support in ProteoWizard, developed for use with Java. Bad
4/16/18
Gautam Saxena
, …
David Bouyssié
3
4/8/18
Q&A
isotopic profile of heavy labelled peptides
Hi Gautam, This is a typical problem when using 15N labeling, the shape of the isotopic pattern of
unread,
Bioinformatics
Q&A
isotopic profile of heavy labelled peptides
Hi Gautam, This is a typical problem when using 15N labeling, the shape of the isotopic pattern of
4/8/18
adriaan...@gmail.com
,
David Bouyssié
3
2/25/18
Q&A
MS1 peak area vs peak height for protein quantification
HI David, Thanks for the answer. The observation I mentioned in my original post was based on data
unread,
label-free
quantitation
Q&A
MS1 peak area vs peak height for protein quantification
HI David, Thanks for the answer. The observation I mentioned in my original post was based on data
2/25/18
viktori...@fh-hagenberg.at
, …
Julian Uszkoreit
11
1/15/18
Q&A
Manual MS2 spectra annotation tool
Hej Viki, Also PIA (in KNIME) and PrideInspector allow for viewing and some annotation of MS/MS
unread,
Annotation
Visualization
Q&A
Manual MS2 spectra annotation tool
Hej Viki, Also PIA (in KNIME) and PrideInspector allow for viewing and some annotation of MS/MS
1/15/18
poori...@gmail.com
, …
Vladimir Gorshkov
4
1/2/18
Q&A
TMT 11-plex
Hi, Vladimir I just saw the spectra already. i see double peaks around 131.138180 and 131.14449. Now,
unread,
Method
TMT
Q&A
TMT 11-plex
Hi, Vladimir I just saw the spectra already. i see double peaks around 131.138180 and 131.14449. Now,
1/2/18
HF
,
Vladimir Gorshkov
3
11/29/17
Q&A
SILAC data analysis
Thanks Vladimir for your thoughtful and quick response. On Wednesday, November 29, 2017 at 10:04:02
unread,
MaxQuant
label-free
Q&A
SILAC data analysis
Thanks Vladimir for your thoughtful and quick response. On Wednesday, November 29, 2017 at 10:04:02
11/29/17
niki...@gmail.com
,
Vladimir Gorshkov
2
11/23/17
Q&A
Isolation interference and MaxQuant
Hi, Nikiana, according to MaxQuant tables description (one can find it in results folder with the
unread,
MaxQuant
Q&A
Isolation interference and MaxQuant
Hi, Nikiana, according to MaxQuant tables description (one can find it in results folder with the
11/23/17
niki...@gmail.com
,
anneg1...@gmail.com
3
11/14/17
Q&A
Isobaric labeling of immunoprecipitation
Hi Anne, Thank you very much for replying and really sorry for my late. Somehow I lost the
unread,
IP
TMT
immunoprecepitation
isobaric-tags
Q&A
Isobaric labeling of immunoprecipitation
Hi Anne, Thank you very much for replying and really sorry for my late. Somehow I lost the
11/14/17