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Genome Engineering using CRISPR/Cas Systems

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Rhiannon Macrae, … Zeki Ilker Kanbagli3

1/13/20

Reminder: Registration is now open for our annual Genome Engineering Workshop!

Hi there Zhang lab! Are you going to arrange Genome Engineering Workshop this year too? Waiting for

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Reminder: Registration is now open for our annual Genome Engineering Workshop!

Hi there Zhang lab! Are you going to arrange Genome Engineering Workshop this year too? Waiting for

1/13/20

Feb 7

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Feb 7

Sharp, … Camilla Bragonzi10

Feb 2

How to calculate MOI based on percent transduction

Hi Neville, I was wondering if when calculating a low MOI based on 30-50% survival after puro

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How to calculate MOI based on percent transduction

Hi Neville, I was wondering if when calculating a low MOI based on 30-50% survival after puro

Feb 2

Jan 30

Postdoc opportunities in genome editing, RNA and cancer

Dear all, I have two Postdoc positions on an exciting project harnessing genome editing to identify

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Postdoc opportunities in genome editing, RNA and cancer

Dear all, I have two Postdoc positions on an exciting project harnessing genome editing to identify

Jan 30

Jan 28

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Jan 15

GecKO CRISPR Screen PCR#1/#2

Hi everyone, I am confused about the PCR steps. I will use MGI platform for the sequencing thats why

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GecKO CRISPR Screen PCR#1/#2

Hi everyone, I am confused about the PCR steps. I will use MGI platform for the sequencing thats why

Jan 15

11/17/25

Need Partners for a legitimate (covert, discreet) transaction!

‎Need Partners for a legitimate (covert, discreet) transaction! ‎Clients wants to move assets ( ~

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Need Partners for a legitimate (covert, discreet) transaction!

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Need Partners for a legitimate (covert, discreet) transaction!

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11/17/25

sambhavi senthil

10/29/25

Recommendations for generating Cas9 expressing cells

Hello everyone, We are planning to conduct an experiment using the LentiCas9-Blast lentiviral prep

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Recommendations for generating Cas9 expressing cells

Hello everyone, We are planning to conduct an experiment using the LentiCas9-Blast lentiviral prep

10/29/25

Camilla Bragonzi

10/27/25

Survival rate is too high in Jurkat transduced with lentivirus

Hi everyone, I am experiencing a very high survival rate when using Jurkat (suspension leukemia T

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Survival rate is too high in Jurkat transduced with lentivirus

Hi everyone, I am experiencing a very high survival rate when using Jurkat (suspension leukemia T

10/27/25

10/2/25

course on Introduction to CRISPR (13–16 Oct, online)!

Hi, There's still time to register for the Physalia course on Introduction to CRISPR for Ecology

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course on Introduction to CRISPR (13–16 Oct, online)!

Hi, There's still time to register for the Physalia course on Introduction to CRISPR for Ecology

10/2/25

8/14/25

Prime editing success?

Hello all, Has anyone had any success trying to use prime editing? I have tried a couple of times,

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Prime editing success?

Hello all, Has anyone had any success trying to use prime editing? I have tried a couple of times,

8/14/25

7/28/25

CRISPR screens in primary T-cells.

Hi all, Does anyone in the group have expertise in conducting CRISPR screens in primary T-cells? I

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CRISPR screens in primary T-cells.

Hi all, Does anyone in the group have expertise in conducting CRISPR screens in primary T-cells? I

7/28/25

7/22/25

Next generation CRISPRko genome-wide libraries from Vivlion GmbH

Hi CRISPR Community, My name is Jessica, and I work in functional genomics and CRISPR screening at

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Next generation CRISPRko genome-wide libraries from Vivlion GmbH

Hi CRISPR Community, My name is Jessica, and I work in functional genomics and CRISPR screening at

7/22/25

7/11/25

gDNA Amplification

Hi All, I have recently extracted genomic DNA from all of my samples and I have got WAY more genomic

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gDNA Amplification

Hi All, I have recently extracted genomic DNA from all of my samples and I have got WAY more genomic

7/11/25

7/7/25

Online Course – Introduction to CRISPR in October

Dear all, We are excited to announce the Introduction to CRISPR for Ecology and Evolution Studies

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Online Course – Introduction to CRISPR in October

Dear all, We are excited to announce the Introduction to CRISPR for Ecology and Evolution Studies

7/7/25

6/18/25

Plasmid request of NovaIscB-ωRNA

Hi everyone, I have to say that NovaIscB is the most exciting project for me recently, because I have

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Plasmid request of NovaIscB-ωRNA

Hi everyone, I have to say that NovaIscB is the most exciting project for me recently, because I have

6/18/25

6/11/25

Hi All, In your expert opinions, which combination of annealing temp and primer conc (nM) would you

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Hi All, In your expert opinions, which combination of annealing temp and primer conc (nM) would you

6/11/25

6/1/25

http://genome-engineering.org/ not accessible

Hi, I tried to access http://genome-engineering.org/ many times today and failed every time. Do you

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http://genome-engineering.org/ not accessible

Hi, I tried to access http://genome-engineering.org/ many times today and failed every time. Do you

6/1/25

Emre Deniz, Neelam Bodhale2

6/1/25

GeCKO website down

Hi I am facing the same issue. Thank you Neelam On Thursday, 12 July 2018 at 04:04:01 UTC-5 emr...@

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GeCKO website down

Hi I am facing the same issue. Thank you Neelam On Thursday, 12 July 2018 at 04:04:01 UTC-5 emr...@

6/1/25

Eduarda Baggio Paglia

5/29/25

Has Anyone Successfully Used pMBP-CRISPR-Cas12a? Looking for Advice

Hi all, My name is Eduarda, and I'm a PhD student in Chemical Engineering. I'm working on a

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Has Anyone Successfully Used pMBP-CRISPR-Cas12a? Looking for Advice

Hi all, My name is Eduarda, and I'm a PhD student in Chemical Engineering. I'm working on a

5/29/25

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