%[Download] Mobi Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition

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Manipulating the Mouse Embryo: A Laboratory Manual, Fourth Edition

by : Richard Behringer

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Book Synopsis : This 4th edition of the "Mouse Manual"-Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have reorganized and updated this edition to include new information and protocols on: . assisted reproduction techniques for sperm and embryo cryopreservation . generation of induced pluripotent stem cells . isolation, generation, and transplantation of spermatogonial stem cell lines . in utero electroporation of gene constructs into post-implantation embryos . vibratome sectioning of live and fixed tissues for imaging thick tissue sections . whole-mount fluorescent staining methods for three-dimensional visualization. Techniques regarding recombinant DNA technology and mouse embryonic development from the previous editions have been updated