In silico PCR error

[Alexis Bennett]

Hello,

I am trying to use the in silico PCR tool to generate amplicon sequences for a CRISPR variant detection analysis. I selected human reference genome build hg19 and input a forward and reverse primer and received the following message that the server was down.

• Error in TCP non-blocking connect() 111 - Connection refused. Host blat1c.soe.ucsc.edu IP 128.114.119.183 port 17917.
  
  Operation now in progress
  Sorry, the BLAT/iPCR server seems to be down. Please try again later: blat1c.soe.ucsc.edu 17917

I received the same error when I tried yesterday. Is there something wrong on my end? Thanks!

Best,

Alexis

[Matthew Speir]

Hello, Alexis.

Thank you for your question about in silico PCR. We are not currently having any issues with the isPCR tool, and the backend server listed in the error message does not match the one we have in our databases.

Can you confirm you are using our main site or one of our official mirrors? They are:

• https://genome.ucsc.edu
• https://genome-euro.ucsc.edu
• https://genome-asia.ucsc.edu

I hope this is helpful. If you have any further questions, please reply to gen...@soe.ucsc.edu. All messages sent to that address are archived on a publicly-accessible Google Groups forum. If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

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Matthew Speir

UCSC Genome Browser, User Support

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