problems uploading bigwig files -- Partial Content 206 error
Nishimura,Erin
Dear UCSC,
I’m having the most frustrating time uploading .bw files to the UCSC genome browser. This has worked for me in the past, and currently the problem is intermittent.
For example, I can successfully upload the file:
http://sites.bmb.colostate.edu/onishlab/Erin/20160613_8cell_average/ABal_average.bw
But when I copy that same file into a neighboring directory, here…
http://sites.bmb.colostate.edu/onishlab/Erin/20170427_elt2elt7/ABal_average.bw
… the file does not upload correctly.
I currently want to upload this .bw file….
but when I do so, I get the following error (below and attached screen shot)…
Error Expected Partial Content 206. http://sites.bmb.colostate.edu/onishlab/Erin/20170427_elt2elt7/JM147_GFPneg_01_02_2014_18_04_2014x50n.bw;byterange=0-: 403 Forbidden. rangeStart=0 rangeEnd=18446744073709551615 Can't get data socket for http://sites.bmb.colostate.edu/onishlab/Erin/20170427_elt2elt7/JM147_GFPneg_01_02_2014_18_04_2014x50n.bw
Any ideas on how to fix this?
Thanks,
Erin.
*************************************************
Erin Nishimura, PhD
Colorado State University
Biochemistry & Molecular Biology
1870 Campus Delivery
200 W. Lake St.
239 MRB Building
Fort Collins, CO 80523
http://www.bmb.colostate.edu/people/erinnish
*************************************************
Christopher Lee
Thank you for your question about loading custom tracks on the Genome
Browser. It appears that the server hosting your bigWig files does not
support byte range requests, but probably did at one point. You must
ask your system admins to (re)enable byte-range support for your
server.
You can test that your server supports byte ranges via the following
curl command to request the first byte:
$ curl -r 0-0 http://sites.bmb.colostate.edu/onishlab/Erin/20160613_8cell_average/ABal_average.bw
Right now, your server returns a 403 error with that request:
$ curl -r 0-0 http://sites.bmb.colostate.edu/onishlab/Erin/20160613_8cell_average/ABal_average.bw
<!DOCTYPE HTML PUBLIC "-//IETF//DTD HTML 2.0//EN">
<html><head>
<title>403 Forbidden</title>
</head><body>
<h1>Forbidden</h1>
<p>You don't have permission to access
/onishlab/Erin/20160613_8cell_average/ABal_average.bw
on this server.</p>
</body></html>
The file that you mentioned as working is likely cached in our system
and so is able to display, while the other files have newer timestamps
and hence we try to make new byte-range requests of the bigWig files,
which then fail because your server does not support the request.
If your system admins are unable to support byte-range requests, you
may be interested in hosting your data at the NSF funded CyVerse. See
this previously answered question for some information about hosting
your data at CyVerse and how to create "Send to Genome Browser" links
that support byte-range requests:
https://groups.google.com/a/soe.ucsc.edu/d/msg/genome/_Ws2jxNfJV4/0c5aGb_PAQAJ
Thank you again for your inquiry and using the UCSC Genome Browser. If
you have any further questions, please reply to gen...@soe.ucsc.edu.
All messages sent to that address are archived on a
publicly-accessible forum. If your question includes sensitive data,
you may send it instead to genom...@soe.ucsc.edu.
Christopher Lee
UCSC Genomics Institute
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