Add .hic file with normalization in UCSC browser

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Marco DI STEFANO

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Sep 4, 2025, 11:40:47 AMSep 4
to gen...@soe.ucsc.edu
To whom this may concern,

I am trying to upload my Hi-C data on UCSC browser and I have problems to show the normalized files.
Briefly, what I did is the following:
1 - I generated .hic files from .allValidPairs file using the following command:
java -Xmx100g -jar ${JUICERJAR} pre ${allValidPairsFile} ${hicFile} ${GSIZE} -r 1000,2000,3000,5000,10000,15000,20000 -j 16 -v
where JUICERJAR=juicer_tools_1.22.01.jar and GSIZE is the file with the chromosome sizes in mm10. 

I am pretty sure this steps worked and that the final .hic file contains KR normalization weigths because if I run the command:
java -jar ${JUICERJAR} dump norm KR ${hicFile} 18 BP 1000
I obtain a list of weights dumped in the terminal as expected.

2 - When I load the file in the UCSC browser, I can see the raw data at all the expected resolution. 
However, if I go to the menu of the file, I cannot find any normalized map in the drop-down menu "Score normalization:", not even "KR" which I know is contained in the file.

3 - Next, I saw that only .hic versions 6-8 are accepted in the USCS browser, but I didn't find any way to downgrade my .hic files or to generate from the $allValidPairsFile a v8 .hic file.

Can you help me to solve this problem?

Thanks in advance for your work.

Best wishes,
Marco Di Stefano

Researcher

Chromatin and Cell Biology Group,

CNRS-Institute of Human Genetics,
Montpellier, France

Gerardo Perez

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Sep 10, 2025, 3:09:30 AMSep 10
to Marco DI STEFANO, gen...@soe.ucsc.edu

Hello, Marco.

Thank you for your interest in the Genome Browser.

Could you share the URL to your HIC file? You can send it to our confidential support list at genom...@soe.ucsc.edu. We currently support HIC files up to version 9. We can check for the various components, such as the file version, byte-range access, etc.

I hope this is helpful. Please include gen...@soe.ucsc.edu in any replies to ensure visibility by the team. All messages sent to that address are archived on a publicly-accessible Google Groups forum.

Gerardo Perez
UCSC Genomics Institute


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