I hope this email finds you well, and thank you for the work that you are doing.
I am trying to view the homologous sequences of BED regions generated using the cattle reference genome ARS-UCD1.2 in the Hainan Bos indicus genome assembly HN024 (ASM3988116v1; GenBank accession: GCA_039881165.1). However, I am unable to find the appropriate chain file on the UCSC Genome Browser website.
The assembly information is as follows:
Assembly name: ASM3988116v1
GenBank accession: GCA_039881165.1
Taxon: Bos indicus (zebu cattle)
Breed: Hainan
Isolate: HN024
WGS project: JAMBMU01
Assembly type: haploid
Submitter: Northwest A&F University
Date: May 29, 2024
I tried to manually create the chain file according to the UCSC pipeline:
https://genomewiki.ucsc.edu/index.php/DoSameSpeciesLiftOver.pl
However, Parasol did not work very well on our computing cluster, especially since I do not have ROOT access and cannot easily solve the related installation and configuration problems.
Therefore, I was wondering if it would be possible to provide a chain file from ARS-UCD1.2 to the Hainan Bos indicus assembly HN024 (ASM3988116v1; GCA_039881165.1). It would also be very helpful if the pipeline could support other job submission systems, such as PBS, QSUB, JSUB, etc.
Thank you for your time, and I look forward to hearing from you.
Best regards,
Xiaoyu Luo
Dear Hiram,
Good afternoon.
Thank you very much for your quick response and for providing the two liftOver chain files. These are exactly what I need.
I really appreciate your help.
Best regards,
Xiaoyu Luo
| From | Hiram Clawson<hcla...@ucsc.edu> |
| Date | 5/18/2026 08:40 |
| To | Xiaoyu Luo<lxy19...@126.com>, gen...@soe.ucsc.edu<gen...@soe.ucsc.edu> |
| Subject | Re: [genome] Request for liftOver chain file from ARS-UCD1.2 to Hainan Bos indicus assembly GCA_039881165.1 |