problem converting a bed to bigBed file

[Theodosiou, Loukas]

Dear UCSC team, 

I highly appreciate your time and support. 

I am relatively new to the UCSCborwser, and I would like some help. 

I aim to upload a new Genome as a track Hub in the UCSC genome browser. 

I have followed the tutorial [here](http://genomewiki.ucsc.edu/index.php/Assembly_Hubs) and [here](https://genome-blog.soe.ucsc.edu/blog/2021/08/03/how-make-a-bigbed-file-part-1/) in detail. 

I am trying to convert my bed file to bigBed, and I keep getting an error that this is not a .bigBed file. I share my data at the [address](https://drive.google.com/drive/folders/1-wmbc9gKtbXFJ95E0n41WgPL-G313SNe?usp=share_link). 

I am using the new _Arabidopsis thaliana_ as a test genome for uploading in the UCSC browser. 

I have downloaded the kent Utilities as it is indicated in the tutorials. 

First I am converting the gff3 file of _Arabidopsis thaliana_  to a bed file, using [agat](https://anaconda.org/bioconda/agat) 

```

agat_convert_sp_gff2bed.pl -gff Arabidopsis_thaliana_TAIR10.gff3 -o arath_gff.bed

```

Then I sort the bed file 

```

sort -k1,1 -k2,2n arath_gff.bed > arath_gff_sorted.bed

```

Lastly, I have used different commands to convert .bed to .bigBed, but none works. 

```

bedToBigBed arath_gff_sorted.bed arath.chrom.sizes arath.bigBed 

```

or 

```

bedToBigBed -type=bed12+ -as=bed12Source.as -tab arath_gff_sorted.bed arath.chrom.sizes arath.bigBed 

```

To evaluate if my .bigBed file is functional, I am using the following command. 

```

bigBedToBed -chrom=chr1 -start=1 -end=10 arath.bigBed stdout

```

Which gives me the following error:

*arath.bigBed is not a big bed file*

Any help or guidance is appreciated!