[genome-announce] CRISPR Targets for human (T2T CHM13v2.0/hs1) now available

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Jairo Navarro Gonzalez

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Feb 8, 2024, 7:44:48 PMFeb 8
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Hello everyone,

We are pleased to announce the release of the CRISPR Targets track for human (T2T CHM13v2.0/hs1).

The CRISPR Targets track shows the DNA sequences targetable by CRISPR RNA guides using the Cas9 enzyme from S. pyogenes (PAM: NGG) over the entire human (hs1) genome. CRISPR target sites were annotated with predicted specificity (off-target effects) and predicted efficiency (on-target cleavage) by various algorithms through the tool CRISPOR. Sp-Cas9 usually cuts double-stranded DNA three or four base pairs 5' of the PAM site.

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