CRISPR Targets for Zebrafish (danRer10/danRer11) now available

[Gerardo Perez]

Hello Everyone,

We are happy to announce the release of the CRISPR Targets track for the Zebrafish danRer10 and danRer11 assemblies. CRISPR-Cas9 has been applied in Zebrafish for gene knockout, gene screening, and other gene editing studies.

The CRISPR Targets track shows the DNA sequences targetable by CRISPR RNA guides using the Cas9 enzyme from S. pyogenes (PAM: NGG) over the entire zebrafish genome. CRISPR target sites were annotated with predicted specificity (off-target effects) and predicted efficiency (on-target cleavage) by various algorithms through the tool CRISPOR. The target sequence of the guide is shown with a thick (exon) bar. The PAM motif match (NGG) is shown with a thinner bar. Guides are colored to reflect both predicted specificity and efficiency. Specificity reflects the "uniqueness" of a 20mer sequence in the genome; the less unique a sequence is, the more likely it is to cleave other locations of the genome (off-target effects). Efficiency is the frequency of cleavage at the target site (on-target efficiency).