Track hub and assembly hub

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Sarah Djebali

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Dec 8, 2017, 11:12:18 AM12/8/17
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Dear all,

I have data for 4 species, 3 whose genome is at UCSC (chicken, cow and pig) and 1 whose genome is not (goat).

I wanted to make an hub that had the 4 species within it, and I apparently managed to make something that passes the hubcheck quality control :
bosTau8/  capHic102/  galGal5/  susScr11/

With this hub I can visualize the 3 genomes that are at UCSC but I do not manage to access the 4th genome that is not although I have made these two files
capHic102/capHic102.2bit  capHic102/capHic102.chrom.sizes

Do you know how to fix the problem? and is it possible at all to do that?

Best,
Sarah
--
***********************************************************
Sarah Djebali Quelen - PhD
INRA GenPhySE, ch. de Borderouge
31326 Castanet-Tolosan, France
sarah.djebali-quelen at inra dot fr

Matthew Speir

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Dec 8, 2017, 6:28:17 PM12/8/17
to Sarah Djebali, gen...@soe.ucsc.edu
Hi Sarah,

Thank you for your question about assembly hubs in the UCSC Genome Browser.

When I connect your hub, it appears to load fine and I'm able to view your hub through the genome gateway page: http://genome.ucsc.edu/cgi-bin/hgGateway. Assemblies from hubs can be found at the top of the list of genomes on the left-hand side of the page.

However, when I attempt to view this genome, I see the error:

    Couldn't open http://genoweb.toulouse.inra.fr/~sdjebali/fragencode/mixhub/2018/groups.txt

at the top of the page. This error indicates that you reference this "groups.txt" file in your genomes.txt file, but the file is not actually present in the directory: http://genoweb.toulouse.inra.fr/~sdjebali/fragencode/mixhub/2018/. You either need to create this file, or remove the reference to it in your genomes.txt file. Since it looks like you don't use custom groups in your trackDb.txt file, you could probably just remove the offending line from your genomes.txt.

One other comment on your hub, for your genome, you should create a new htmlPath path page. The current one, which looks to just be a copy of an NCBI page, doesn't interact well with Javascript and HTML already present on the page. You can see this here: http://genome.ucsc.edu/cgi-bin/hgGateway?genome=capHic102&hubUrl=http://genoweb.toulouse.inra.fr/~sdjebali/fragencode/mixhub/2018/hub.txt. A good example of an assemblyl description page can be found in the C_elegans_isolates assembly hub here: http://waterston.gs.washington.edu/trackhubs/isolates/CB4856Princeton_Contigs/description.html.

I hope this is helpful. If you have any further questions, please reply to gen...@soe.ucsc.edu. All messages sent to that address are archived on a publicly-accessible Google Groups forum. If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

Matthew Speir
UCSC Genome Bioinformatics Group
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Sarah Djebali

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Dec 11, 2017, 11:21:10 AM12/11/17
to Matthew Speir, gen...@soe.ucsc.edu
Dear Matthew,

Thanks a lot for your fast and detailed answer.

I removed the row
groups groups.txt
from the genomes.txt file, 

however I still cannot see the goat genome when I type "cap..." in the white box on the top left of the page, above the species tree, should I access it in a different way? do you still see an error message?

I will fix the html issue afterwards.

Best,
Sarah

On Sat, Dec 9, 2017 at 12:28 AM, Matthew Speir <msp...@soe.ucsc.edu> wrote:
Hi Sarah,

Thank you for your question about assembly hubs in the UCSC Genome Browser.

When I connect your hub, it appears to load fine and I'm able to view your hub through the genome gateway page: http://genome.ucsc.edu/cgi-bin/hgGateway. Assemblies from hubs can be found at the top of the list of genomes on the left-hand side of the page.

However, when I attempt to view this genome, I see the error:

    Couldn't open http://genoweb.toulouse.inra.fr/~sdjebali/fragencode/mixhub/2018/groups.txt

at the top of the page. This error indicates that you reference this "groups.txt" file in your genomes.txt file, but the file is not actually present in the directory: http://genoweb.toulouse.inra.fr/~sdjebali/fragencode/mixhub/2018/. You either need to create this file, or remove the reference to it in your genomes.txt file. Since it looks like you don't use custom groups in your trackDb.txt file, you could probably just remove the offending line from your genomes.txt.

One other comment on your hub, for your genome, you should create a new htmlPath path page. The current one, which looks to just be a copy of an NCBI page, doesn't interact well with Javascript and HTML already present on the page. You can see this here: http://genome.ucsc.edu/cgi-bin/hgGateway?genome=capHic102&hubUrl=http://genoweb.toulouse.inra.fr/~sdjebali/fragencode/mixhub/2018/hub.txt. A good example of an assemblyl description page can be found in the C_elegans_isolates assembly hub here: http://waterston.gs.washington.edu/trackhubs/isolates/CB4856Princeton_Contigs/description.html.

I hope this is helpful. If you have any further questions, please reply to gen...@soe.ucsc.edu. All messages sent to that address are archived on a publicly-accessible Google Groups forum. If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

Matthew Speir
UCSC Genome Bioinformatics Group



On 12/7/17 11:38 PM, Sarah Djebali wrote:
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Jairo Navarro Gonzalez

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Dec 18, 2017, 1:31:35 PM12/18/17
to Sarah Djebali, gen...@soe.ucsc.edu

Hello Sarah,

Thank you for using the UCSC Genome Browser and your follow-up question.

I am no longer seeing the error and am able to load your assembly hub into the genome browser. You can access your goat assembly by going to the top of the list of genomes on the left-hand side of the gateway page. Once at the top of the list, you will see your assembly hub:

FR-AgENCODE 2018

Selecting this hub, you will be able to display your goat assembly by clicking Go on the gateway page. Unfortunately, searching for capHic102 will not find your user uploaded goat assembly.

I hope this is helpful. If you have any further questions, please reply to gen...@soe.ucsc.edu.
All messages sent to that address are archived on a publicly-accessible Google Groups forum.
If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

Jairo Navarro 
UCSC Genomics Institute

Want to share the Browser with colleagues?
Host a workshop: http://bit.ly/ucscTraining


Sarah Djebali

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Dec 19, 2017, 11:50:42 AM12/19/17
to Jairo Navarro Gonzalez, gen...@soe.ucsc.edu
Hi Jairo,

Thanks a lot I can now see the goat genome, and it is really nice!

Best,
Sarah

On Mon, Dec 18, 2017 at 7:31 PM, Jairo Navarro Gonzalez <jnav...@ucsc.edu> wrote:

Hello Sarah,

Thank you for using the UCSC Genome Browser and your follow-up question.

I am no longer seeing the error and am able to load your assembly hub into the genome browser. You can access your goat assembly by going to the top of the list of genomes on the left-hand side of the gateway page. Once at the top of the list, you will see your assembly hub:

FR-AgENCODE 2018

Selecting this hub, you will be able to display your goat assembly by clicking Go on the gateway page. Unfortunately, searching for capHic102 will not find your user uploaded goat assembly.

I hope this is helpful. If you have any further questions, please reply to gen...@soe.ucsc.edu.


All messages sent to that address are archived on a publicly-accessible Google Groups forum.
If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

Jairo Navarro 
UCSC Genomics Institute

Want to share the Browser with colleagues?
Host a workshop: http://bit.ly/ucscTraining

On Mon, Dec 11, 2017 at 2:34 AM, Sarah Djebali <sarah.djeb...@inra.fr> wrote:
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