Exon sequence

[Blokhin, Ilya O]

Good morning.

How can we extract the sequence of a specific exon of specific gene? We are interested in exon 17 or human EGFR.

Thanks,

Ilya

[Robert Kuhn]

Hello, Ilya,

The short version of getting the sequence of any region is to zoom to

exactly that part of the genome in the main Browser graphic, then use the

pulldown menu, "View" and select "DNA."

A longer, video version can be found via a link on our training page:

  http://genome.ucsc.edu/training/

Obtaining exon coordinates and sequences.

https://www.youtube.com/watch?v=6JoUqM1iKxQ&list=UUQnUJepyNOw0p8s2otX4RYQ

best regards, and thanks for using the Genome Browser,

  --b0b kuhn

   ucsc genome bioinformatics group 

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[Robert Kuhn]

Hello, again, Ilya,

I would like to add that you should be careful when using "exon 17" that you

are in the right place on the gene.  Because of differential splicing there are

different isoforms and "exon 17" will be different depending on the isoform you

choose.  

regards,

 --b0b kuhn

[Blokhin, Ilya O]

Thanks, Bob