bug at In Silico PCR

[Wayne M. Barnes]

"If you are looking for matches to RT-PCR primers, where primers often straddle intron-exon boundaries, change the Target option and select a gene transcript set."

There is no Target option for any gene transcript set. 

Thank you for your help,  I would like to In Silico PCR for the human transcriptome, which is a misnomer for all of the spliced mRNA sequences, listed as DNA.

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Dr. Wayne M. Barnes, Ph.D.
Biochemistry Dept. 8231
Washington Univ. Medical School
660 South Euclid Ave.  [Shipping: 4523 Clayton Ave]  [Uber: 4565 McKinley]
St. Louis, MO 63110   
Lab: McDonnell 212
ph:  314.362.3351    fax:  314.754.9556   text: 31468...@txt.att.net
http://barnes1.wustl.edu wa...@barnes1.wustl.edu

[Jairo Navarro Gonzalez]

Hello,

Thank you for using the UCSC Genome Browser and reporting your issues.

Could you share which assembly you are using on the UCSC Genome Browser to help reproduce your issues? The text on the isPCR is generic and is displayed regardless of the assembly you have selected. Changing the target from the 'genome assembly' to a gene track is only available for human and mouse genome assemblies.

Unfortunately, if you are using the hs1 assembly for human, this feature is not yet available.

I hope this is helpful. If you have any further questions, please reply to gen...@soe.ucsc.edu.
All messages sent to that address are archived on a publicly accessible Google Groups forum.
If your question includes sensitive data, you may send it instead to genom...@soe.ucsc.edu.

Jairo Navarro
UCSC Genome Browser

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