Fwd: EMBO - ukázka superrezolučních technik
Mgr. Ondřej Šebesta
Univerzita Karlova v Praze
Přírodovědecká fakulta
Laboratoř Konfokální a Fluorescenční mikroskopie
Viničná 7, 128 44 Praha 2
Telefon: +420 22195 1061
E-mail: ondrej....@natur.cuni.cz
Charles University in Prague
Faculty of Science
Albertov 6, 128 43 Praha 2
www.natur.cuni.cz/en
Od: Ondrej Hovorka Ph.D. <ohov...@svenbiolabs.cz>
I would like to offer you the opportunity to test the super-resolution systems that will be installed as part of the EMBO Super-Resolution Course in Prague at the Institute of Molecular Genetics of the Czech Academy of Sciences.
Admission to the EMBO course is rather competitive; however, this year two systems from our distributor partners will be on site, and we have arranged that, during the participants’ theoretical lectures, you’ll be able to see and try the instruments firsthand with the manufacturers’ experts.
I can offer you a time slot on Friday, June 20, from 9:00 AM to 1:00 PM, with two hours dedicated to the Abbelight system and two hours to the CSR Biotech system.
You are welcome to bring your own samples to test on both systems, although we will also provide demonstration specimens, so it’s not strictly necessary. If you do wish to prepare your own slides, note that for the Abbelight system you’ll need an online discussion with the manufacturer in advance, since sample preparation for their system requires some practice with SMLM. Please let me know if you plan to bring your own samples and whether I may share your email address with Abbelight so they can schedule a preparatory online meeting with you.
Below are links to the two super-resolution systems mentioned:
Abbelight Nanoscopy
https://www.abbelight.com/technology/nanoscopy/
Nanoscopy—also known as Single-Molecule Localization Microscopy (SMLM)—circumvents the diffraction limit of fluorescence microscopy to deliver nanometric spatial super-resolution. This revolutionary breakthrough employs photoswitchable fluorescent dyes that can be toggled between emitting and non-emitting states, enabling precise localization of individual molecules with nanometer accuracy (10 × 10 nm in XY and 15 nm along the XZ axes). By its very principle, SMLM not only achieves such remarkable super-resolution but also allows quantification and counting of single molecules in biological specimens.
CSR Biotech MI-SIM
https://www.csr-biotech.com/en/home
MI-SIM offers a comprehensive super-resolution microscopy solution for rapid imaging of dynamic processes in living cells. With exceptional spatial resolution down to 60 nm, imaging speeds of up to 1,500 frames per second, and a large 150 µm field of view using a 100× objective, MI-SIM sets new standards for 3D super-resolution imaging of various live-cell dynamics. Its advanced sensitivity and reduced phototoxicity facilitate long-term imaging while minimizing photobleaching effects.
I believe this is a fantastic opportunity to see these high-end technologies in action, and we would be delighted to welcome you to this event.
Wishing you all the best and looking forward to your reply.
Kind regards,
Ondra Hovorka
