IMG/MER and preprocessing

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Lisa

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Dec 2, 2016, 2:27:57 PM12/2/16
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I have illumina shotgun sequencing data (Nextseq) that I would like to upload to IMG. The fastq sequences are contained in 8 files per sample (the samples were spread out on 4 lanes, and they are paired-end reads). Does IMG put together the paired ends? Is it best to submit the fastQ files for the 4 lanes separately? Is it better to do pre-processing/assembly first  with other programs before submission?

Thank you for any advice. 

I-Min Chen

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Dec 2, 2016, 4:13:04 PM12/2/16
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IMG does not perform assembly. Therefore, you will need to assemble your own sequence data. We only accept fasta (i.e., no fastq).
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