Hello,
I submitted 13 metagenomes back in January that were in the batch that had to be re-run due to the incorrect KO/EC assignmen. Now I am looking at the newly re-run data and the percent of hits to the KO database is still extremely low. The percent of genes hitting COG and pfam runs between 50-70%, were as KO is 1-3%. For previously submitted metagenomes these percentages were nearly equal, so it seems very odd that for this batch of metagenomes the percentage of KO assignments are so low.
If anyone could lend insight into why this might be that would be extremely helpful. Thank you,
-Caroline