Hi Ian
thank you for helping.
please see below what I got:
conda install anaconda-client
Fetching package metadata ...............
Solving package specifications: .
# All requested packages already installed.
# packages in environment at /Users/jdhahbi/miniconda2:
#
anaconda-client 1.6.3 py27_0
josephs-Mac-Pro:Tophat jdhahbi$ anaconda upload boost_env.yml
Using Anaconda API:
https://api.anaconda.orgThe action you are performing requires authentication, please sign in:
Using Anaconda API:
https://api.anaconda.orgUsername:
(It is asking for a Username)
conda env export > boost_env.yml
CondaEnvException: Conda Env Exception: Unable to determine environment
Please re-run this command with one of the following options:
* Provide an environment name via --name or -n
* Re-run this command inside an activated conda environment.
here is the command I use to analyze my data:
tophat --library-type fr-firststrand -r 200 --b2-very-fast --read-realign-edit-dist 0 --transcriptome-index=/Users/jdhahbi//indexes/RefSeq_refFlat_hg19_index -o tophat_out_test /Users/jdhahbi//indexes/hg19/bt2//bt2_hg19 test_pair1_ATCACG_paired.fq test_pair2_ATCACG_paired.fq
Thanks