I ran a scan to see how many third_party PRESUBMIT.py files don't have the "USE_PYTHON3 = True" marker, in hopes that we could soon retire support for Python 2 presubmits in depot_tools (Chromium itself has been Python 3 only in presubmits for quite a while). My script found these seven PRESUBMIT.py scripts:
third_party\angle\infra\config\PRESUBMIT.py
third_party\depot_tools\tests\PRESUBMIT.py
third_party\perfetto\PRESUBMIT.py
third_party\perfetto\test\trace_processor\PRESUBMIT.py
third_party\perfetto\ui\PRESUBMIT.py
third_party\swiftshader\third_party\SPIRV-Tools\PRESUBMIT.py
third_party\vulkan-deps\spirv-tools\src\PRESUBMIT.py
The perfetto team just needed some pointers on how to do the switch and has created a CL that should get all of their presubmits onto Python 3. The angle team created a CL for infra\config\PRESUBMIT.py and another one for SPIRV-Tools\PRESUBMIT.py (which should make it to both copies of that file).
So, once those changes have rolled in then depot_tools\tests\PRESUBMIT.py should be the only PRESUBMIT.py file in a Chromium repo that is missing the tag, and presumably that is only that way in order to support all the other PRESUBMIT.py files.
I don't know what other infra/internal repos there might be, but I've seen a lot of work in that area recently. There might also be external projects using depot_tools presubmit system, but it seems like we might be able to drop support for Python 2 presubmits real-soon-now. It's kind of exciting!