New bacteria strains found in prostatitis patients :UT
Anonymous User
November 1, 2001
By: Mac Overmyer
Urology Times
Whether strains are related to clinical effects and symptoms awaits further
study
Toronto-A team of Canadian researchers has isolated at least two unexpected
strains of bacteria from the genitourinary fluids of men with type III
(nonbacterial) prostatitis. The two strains, Proteobacterium species and
Paenibacillus species, have never before been identified in patients with
prostatitis.
"It [Paenibacillus] was identified in Norwegian spruce trees. We also found it
in one case in the literature, a case of osteomyelitis, but this is the first
time it has been identified in the urinary tract in man," said Keith Jarvi, MD,
associate professor of urology at the University of Toronto and Mount Sinai
Hospital, Toronto. "It is interesting that this is the most common
bacteria we found. This is quite novel."
The questions now facing researchers are whether the bacteria have clinical
effects and whether they are directly or indirectly related to prostatitis
symptomatology.
"This is just an association," Dr. Jarvi said in a presentation at the AUA
annual meeting. "We are not saying that it can cause prostatitis. It appears to
be a common bacteria, and it may just be that the microenvironment of the
disease is conducive to the growth of this bacteria.
"I would underscore that we don't know what is going on with these patients,
but to find something that has never before been found in the semen and urine
of these patients is interesting. I think we may have to go right back to the
basics to find out if these bacteria are important in the condition."
Less prevalent in controls
The theory that unidentified bacteria may be related to the etiology of the
disease has been proposed by others, and culturing bacteria from prostatitis
and many other diseases remains a significant challenge, according to Lori
Burrows, PhD, of the Centre for Infection and Biomaterials Research, Toronto
General Research Institute, who also collaborated on the study. That challenge
is being met by new molecular biology techniques and technologies that allow
researchers to circumvent obstacles by attempting to create cultures for the
bugs.
To identify the bacteria, the research team obtained semen specimens and urine
samples from the early (VB1), midstream (VB2), and endstream (VB3) of 23 men
diagnosed with category III prostatitis and eight controls. The samples were
centrifuged to pellets, which were then lysed.
Segments of DNA were obtained using restriction enzymes. The DNA fragments were
amplified with PCR techniques. The resulting DNA codes were compared with those
in a national database in Urbana, IL. This allowed the team to identify species
of Proteobacterium, and Paenibacillus, as well as Flavobacterium.
In addition to identifying the bacteria for the first time, the team also found
that Paenibacillus was far more common in men with prostatitis than the
controls.
Larger study needed
The next steps in the endeavor are reasonably clear, according to Dr. Jarvi.
"We need to confirm these findings with a larger study," he told Urology Times.
"But to prove that it is causal, an antibiotic that would eradicate it
needs to be identified and applied to see if symptoms are altered.
"In theory, quinolones might work, but we need to find the appropriate culture
first. We have not cultured it out yet," he said.
In addition to identifying novel organisms in prostatitis, Dr. Burrows noted
that another important aspect of the research was that it showed that new
technology can now detect micro-organisms that had escaped notice because they
could not be cultured
Reggie Guy
"Anonymous User" <anon...@remailer.havenco.com> wrote in message
news:67132405b0ee4f3c...@remailer.havenco.com...
>
> "It [Paenibacillus] was identified in Norwegian spruce trees.
(snip)
Talk about "Wood". Perhaps Dr. Garlick should come back to discuss this
since he's from Norway (or was it Sveden?).
Reg.
NiceShyMiamiGuy
Poster Session 4, June 2, 3:30-5:30, 204 A-C
The etiology of National Institutes of Health type III nonbacterial prostatitis
is undetermined, but it is widely believed that bacterial infection may be a
contributing factor. It remains difficult or impossible to culture most
microbial organisms, although culturing techniques have been available for more
than 100 years. Because molecular biology has made it possible to identify the
genotype of bacteria rather than the phenotype, any microorganism may now be
detected, even those that cannot be cultured. These researchers used the new
molecular biological techniques to determine the types of bacteria present in
the semen and urine samples of 23 men with type III prostatitis and 8 men
without prostatitis. They performed polymerase chain reaction and compared the
bacterial sequences obtained with those on deposit in the ribosomal database in
Urbana, Illinois. The DNA of certain unusual bacteria was detected in all of
the semen samples and in 47 of the 58 urine samples, including Paenibacillus
sp., Proteobacterium sp., Flavobacterium, uncultured eubacterium and
Bradyrhizobium sp. Paenibacillus and Proteobacterium were the most common
types, and they were much more common in the men with prostatitis than in the
controls. This is the first reported finding of Paenibacillus sp. or
Proteobacterium sp. in human genitourinary tract fluids. It is surprising that
these 2 previously undetected species should be the most common bacteria in the
semen and urine of the men with type III prostatitis. There was a clear
preponderance of these 2 types in the prostatitis patients compared with the
controls, suggesting that these bacteria are associated with prostatitis. This
association may be causative or the prostatic microenvironment in men with
prostatitis may be conducive to the growth of these specific organisms.
http://www.hisandherhealth.com/aua2001/list.php?category=male
NiceShyMiamiGuy
>> "It [Paenibacillus] was identified in Norwegian spruce trees.
>
>(snip)
>
>Talk about "Wood". Perhaps Dr. Garlick should come back to discuss this
>since he's from Norway (or was it Sveden?).
>
>Reg.
He will not be back as I have banned him from this news group -- next ----->
AndrewsFunk
>He will not be back as I have banned him from this news group -- next ----->
I'd be interested in how you come to such an intimate knowledge ? Are you
psychic, do you hold seances? Are you sick?
The "Ugly American" reapears...online!
NiceShyMiamiGuy
>From: andre...@aol.com (AndrewsFunk)
>Date: 2/25/02 12:48 AM Eastern Standard Time
>Message-id: <20020225004851...@mb-ch.aol.com>
See the way it works is I tell him to stop posting and he stops posting -
simple.
I'd rather be an "Ugly American" than a good looking Canadian - ha ha - my mom
tells me I"m good looking -- ahahahaha!!!
vv
wrote:
Either way, you're still an idiot.
AndrewsFunk
>mom
>>tells me I"m good looking -- ahahahaha!!!
Who said I am Canadian? As for your mother.....she just tells you you're good
looking just to try to get you out of the house to find a girl. But I forgot.
Women are evil. Sorry.