Source for 5-methycytosine monophosphate

[Jim Broker]

I am currently doing student research at Millersville University.
I am running samples of digested chlorella virus and testing them for
percentages of 5-methylcytosine and 6-methyladenine. One of the
standards I need is 5-methylcytosine monophosphate. I am having a hard
time finding it and I was wondering if anybody out there knew a source
for it. If you do, please email me at: jbr...@junglemate.com

any suggestions would be very helpful!

[Tobias Kind]

jbr...@junglemate.com (Jim Broker) wrote in message news:<1c6cebf2.02052...@posting.google.com>...

> I am currently doing student research at Millersville University.
> I am running samples of digested chlorella virus and testing them for
> percentages of 5-methylcytosine and 6-methyladenine. One of the
> standards I need is 5-methylcytosine monophosphate. I am having a hard

Hi,
you don't need the phosphate for your HPLC method.
Go for 5-Methylcytosine hydrochloride, CAS: 58366-64-6
and adjust the pH via buffer.

58366-64-6 is from ABCR, Chimex and Synchem.

Kind regards
Tobias Kind
www.amdis.net

[Des O'Connor]

Hi Jim

I have checked our suppliers (UK) and they only supply the Hydrochloride of
the cytosine molecule you mention. It seems to me that you are going to have
a tough search on this one.

.

How are you performing the assay? If you are using UV I doubt that the
phosphate form would have significantly different characteristics from the
Hdrochloride form in solution.

Try an assay that targets actual neucleotide. If you have to end up
compromising on the chemical form of the standard at least you will have
significant comparative data from your various treatments all other things
being equal etc.

Good luck

des

"Jim Broker" <jbr...@junglemate.com> wrote in message
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