Prozac kills candida!??!?
JRStern
Now, if it would cure athelete's foot, ...
J.
Randall
> http://dailynews.yahoo.com/h/nm/20020121/hl/fungus_1.html
>
> Now, if it would cure athelete's foot, ...
>
> J.
Hi,
I wonder if this was in vitro. As in vivo we'd have more
ancedotes by now. Or are any little rashes on depressed types
only written off as stress remissions?
The article didn't give many clues. I wonder if we can get
the paper off the scientific site?
I remember someone in the p ng who went round and round on
whether taking SRUT's for p was a proper off label use.
Gosh, maybe he meant to take the tabs powderize em and apply
topically. If so. Someone may need to appologize for p's sake.
Are you topical p peoples listening?
(At this point i may add my own personal touch... get it?)
That was for only one persons eyes and he, i hope, knows
who i mean?.
An AD for it, may go something like this.
Is your skin under stress? Flakee? Use all new ProZap to
mellow it all out. warning- only topical.
Do not consume without a Rx from some other MD.
Over the counter? I doubt it... as kids may have another friday nite
high. Take prozap (eat it) and drink XXX, for a great mellow time.
H'mmmmm, i may try it to.
randall... Got P? get ProZap._ mellow out that flake..
JRStern
>Over the counter? I doubt it... as kids may have another friday nite
>high. Take prozap (eat it) and drink XXX, for a great mellow time.
St John's (anti-)Wort?
J.
mike
when they take these drugs because the yeasts are starving for food..
"JRStern" <JRS...@gte.net> wrote in message
news:3c4da82d...@news.gte.net...
Randall
What Wort?
Oh. Yes.
JR's ST Johns Wort!
Mix with 5HTP? And dream the P away?
You know i could have streamed it. I'm in pain
right now and my stream is itching to get out.
CARP oh Tunnel pain too... ouch.
Maybe i need a mirror BOX to trick my Mind.
Red herrings are a real mind field.
Not working, so i'll post my favorite wort post for P.
Its in my mail box.. hold on..B right BACK. ouch don't remind me.
&&&&&&&&&&&&&&&&&& (hey JR have you used it? for ??? &&& PPP ???)
Groups search result 13 for skin cell proliferation
>
> Search Result 13
> From: BiGol...@aol.com (BiGol...@aol.com)
> Subject: St. John's wort inhibits cytochrome P450s & T cell proliferation
> Newsgroups: sci.med.aids
> View: Complete Thread (4 articles) | Original Format
> Date: 2000/07/05
>
>
> Interesting articles just published (7/00 & 5/00) concerning St. John's wort.
>
>
> St John's wort (Hypericum perforatum) is used for the treatment of
> HIV/AIDS, depression, and skin conditions. It is a potent inhibitor of
> cytochrome P450s and inhibits the proliferation of T lymphocytes.
>
> Obach (2000) state: "Commercially available St. John's wort (Hypericum
> perforatum) extracts, preparations that are used in the treatment of
> depression, were examined for the potential to inhibit human cytochrome
> P450 (CYP) enzyme activities, specifically CYP1A2, CYP2C9, CYP2C19,
> CYP2D6, and CYP3A4. Crude extracts demonstrated inhibition of each of
> these five enzymes, with CYP2D6, CYP2C9, and CYP3A4 being more sensitive
> than CYP1A2 and CYP2C19," and "These in vitro data indicate that St.
> John's wort preparations contain constituents that can potently inhibit
> the activities of major human drug-metabolizing enzymes and suggest that
> these preparations should be examined for potential pharmacokinetic drug
> interactions in vivo."
>
> Schempp et al. (2000) state: "St John's wort (Hypericum perforatum) is a
> traditional herbal medicine that is used for the topical treatment of
> superficial wounds, burns and dermatitis," and "The results demonstrate
> an inhibitory effect of Hypericum extract and of its metabolite
> hyperforin on the MECLR [mixed EC lymphocyte reaction] and on the
> proliferation of T lymphocytes that may provide a rationale for the
> traditional treatment of inflammatory skin disorders with Hypericum
> extracts."
>
> ================================
>
> Obach RS. Inhibition of human cytochrome P450 enzymes by constituents of
> st. John's wort, an herbal preparation used in the treatment of
> depression. J Pharmacol Exp Ther 2000 Jul;294(1):88-95.
>
> Drug Metabolism Department, Candidate Synthesis, Enhancement, and
> Evaluation, Central Research Division, Pfizer, Inc., Groton,
> Connecticut.
>
> Abstract: Commercially available St. John's wort (Hypericum perforatum)
> extracts, preparations that are used in the treatment of depression,
> were examined for the potential to inhibit human cytochrome P450 (CYP)
> enzyme activities, specifically CYP1A2, CYP2C9, CYP2C19, CYP2D6, and
> CYP3A4. Crude extracts demonstrated inhibition of each of these five
> enzymes, with CYP2D6, CYP2C9, and CYP3A4 being more sensitive than
> CYP1A2 and CYP2C19. Extracts were fractionated by HPLC, and each of the
> fractions was tested for inhibition of these five CYPs to identify
> individual constituents with inhibitory activity. Several fractions
> were shown to possess inhibitory activity, including the fractions
> containing hyperforin (the putative active antidepressant constituent),
> I3,II8-biapigenin, and hypericin. Hyperforin and I3,II8-biapigenin
> were isolated from the extract, and inhibition constants for the
> five CYP activities were measured. In addition, three other
> constituents, hypericin, quercetin, and chlorogenic acid, were tested
> for inhibitory activity toward the CYP enzymes. The flavonoid compound
> I3,II8-biapigenin was shown to be a potent, competitive inhibitor of
> CYP3A4, CYP2C9, and CYP1A2 activities with K(i) values of 0.038, 0.32,
> and 0.95 &mgr;M, respectively. Hyperforin was a potent noncompetitive
> inhibitor of CYP2D6 activity (K(i) = 1.5 &mgr;M) and competitive
> inhibitor of CYP2C9 and CYP3A4 activities (K(i) = 1.8 and 0.48 &mgr;M,
> respectively). Hypericin also demonstrated potent inhibition of several
> CYP activities. These in vitro data indicate that St. John's wort
> preparations contain constituents that can potently inhibit the
> activities of major human drug-metabolizing enzymes and suggest that
> these preparations should be examined for potential pharmacokinetic drug
> interactions in vivo.
>
> ================================
>
> Schempp CM, Winghofer B, Ludtke R, Simon-Haarhaus B, Schopf E, Simon JC
> Topical application of St John's wort (Hypericum perforatum L.) and of
> its metabolite hyperforin inhibits the allostimulatory capacity of
> epidermal cells. Br J Dermatol 2000 May;142(5):979-84.
>
> Department of Dermatology, Photodermatology Unit, University Medical
> Center, Haupstr 7, D-79104 Freiburg, Germany.
> Email: sch...@haut.uni-freiburg.de
>
> Abstract: St John's wort (Hypericum perforatum) is a traditional herbal
> medicine that is used for the topical treatment of superficial wounds,
> burns and dermatitis. The characteristic metabolites of St John's wort
> are the photodynamic active plant pigment hypericin and the
> phloroglucin-derivative hyperforin. To date, no studies on
> immunomodulatory properties of topical preparations of St John's wort
> have been performed. Here, we investigated the alloantigen presenting
> function of human epidermal cells (EC) exposed to Hypericum ointment in
> vivo in a mixed EC lymphocyte reaction (MECLR). The effect of Hypericum
> ointment was compared with the immunosuppressive effect of solar-
> simulated radiation (SSR). Subsequently, we tested purified hyperforin
> in vivo and in vitro in a MECLR to evaluate its possible contribution to
> the effect of the Hypericum ointment. Furthermore, we assessed the
> effect of hyperforin on the proliferation of peripheral blood
> mononuclear cells (PBMC) in vitro. Compared with untreated skin,
> treatment with Hypericum ointment resulted in a significant suppression
> of the MECLR (P </= 0.001) that was similar to the effect of SSR. The
> combination of Hypericum ointment plus SSR was not significantly
> different from either treatment alone. EC isolated from skin treated
> with the hyperforin containing ointment also showed a reduced capacity
> to stimulate the proliferation of allogeneic T cells (P </= 0.001).
> Similarly, in vitro incubation of EC with hyperforin suppressed the
> proliferation of alloreactive T cells (P </= 0.001). Furthermore,
> hyperforin inhibited the proliferation of PBMC in a dose-dependent
> manner, without displaying pronounced toxic effects as determined by
> Trypan blue staining. The results demonstrate an inhibitory effect of
> Hypericum extract and of its metabolite hyperforin on the MECLR and on
> the proliferation of T lymphocytes that may provide a rationale for the
> traditional treatment of inflammatory skin disorders with Hypericum
> extracts.
>
randall... i liked it.. how come i never opened it.. oh yeah. i sent it.
JRStern
>Not working, so i'll post my favorite wort post for P.
>Its in my mail box.. hold on..B right BACK. ouch don't remind me.
>
>&&&&&&&&&&&&&&&&&& (hey JR have you used it? for ??? &&& PPP ???)
Nope. I think I'm the only one in L.A. not on psychoactive drugs,
legal or illegal, prescription or herbal, ... I should color what hair
I have left, get some piercings, an SUV, a PDA, and a development deal
for my script, I'd fit in better.
J.
Randall
> JRS...@gte.net (JRStern) wrote in message news:<3c4dee63...@news.gte.net>...
> > On 22 Jan 2002 13:54:33 -0800, ranh...@aol.com (Randall) wrote:
> > >Over the counter? I doubt it... as kids may have another friday nite
> > >high. Take prozap (eat it) and drink XXX, for a great mellow time.
> >
> > St John's (anti-)Wort?
I wonder what this means... i remembered an old post on
P-450 and grapefruit juice.. i will post it where it
makes sense to. Below and not far.
> >
> > J.
>
>
> What Wort?
>
> Oh. Yes.
>
> JR's ST Johns Wort!
>
> Mix with 5HTP? And dream the P away?
>
> You know i could have streamed it. I'm in pain
> right now and my stream is itching to get out.
> CARP oh Tunnel pain too... ouch.
> Maybe i need a mirror BOX to trick my Mind.
> Red herrings are a real mind field.
You may want a mime next time. Sorry, couldn't resist.
******************************************
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Grapefruit juice post-translationally inhibits the action of
cytochrome
P450 3A4 (also called CYP3A4), but they are not sure which component
does it. Since this enzyme also helps to metabolize many xenobiotics
and
toxic compounds for excretion, I have decided to reduce my intake of
grapefruit and juices. A damned shame because I love them and they are
low glycemic fruit with red grapefruit even containing the valuable
lycopene!
Here is a review abstract from last August
Br J Clin Pharmacol 1998 Aug;46(2):101-10
Grapefruit juice-drug interactions.
Bailey DG, Malcolm J, Arnold O, Spence JD
Department of Medicine, London Health Sciences Centre, Ontario,
Canada.
The novel finding that grapefruit juice can markedly augment oral drug
bioavailability was based on an unexpected observation
from an interaction study between the dihydropyridine calcium channel
antagonist, felodipine, and ethanol in which grapefruit
juice was used to mask the taste of the ethanol. Subsequent
investigations showed that grapefruit juice acted by reducing
presystemic felodipine metabolism through selective post-translational
down regulation of cytochrome P450 3A4 (CYP3A4)
expression in the intestinal wall. Since the duration of effect of
grapefruit juice can last 24 h, repeated juice consumption can
result in a cumulative increase in felodipine AUC and Cmax. The high
variability of the magnitude of effect among individuals
appeared dependent upon inherent differences in enteric CYP3A4 protein
expression such that individuals with highest
baseline CYP3A4 had the highest proportional increase. At least 20
other
drugs have been assessed for an interaction with
grapefruit juice. Medications with innately low oral bioavailability
because of substantial presystemic metabolism mediated by
CYP3A4 appear affected by grapefruit juice. Clinically relevant
interactions seem likely for most dihydropyridines, terfenadine,
saquinavir, cyclosporin, midazolam, triazolam and verapamil and may
also
occur with lovastatin, cisapride and astemizole. The
importance of the interaction appears to be influenced by individual
patient susceptibility, type and amount of grapefruit juice
and administration-related factors. Although in vitro findings support
the flavonoid, naringin, or the furanocoumarin,
6',7'-dihydroxybergamottin, as being active ingredients, a recent
investigation indicated that neither of these substances made a
major contribution to grapefruit juice-drug interactions in humans.
And here is the most recent one on medline which shows that they still
do not know the full mechanism.
Clin Pharmacol Ther 1999 Mar;65(3):237-44
6',7'-Dihydroxybergamottin in grapefruit juice and Seville orange
juice:
effects on cyclosporine disposition, enterocyte CYP3A4, and
P-glycoprotein.
Edwards DJ, Fitzsimmons ME, Schuetz EG, Yasuda K, Ducharme MP,
Warbasse
LH, Woster PM, Schuetz JD, Watkins P
College of Pharmacy and School of Medicine, Wayne State University,
Detroit, Mich, USA.
BACKGROUND: 6',7'-Dihydroxybergamottin is a furanocoumarin that
inhibits
CYP3A4 and is found in grapefruit juice and
Seville orange juice. Grapefruit juice increases the oral
bioavailability of many CYP3A4 substrates, including cyclosporine
(INN, ciclosporin), but intestinal P-glycoprotein may be a more
important determinant of cyclosporine availability.
OBJECTIVES: To evaluate the contribution of 6',7'-dihydroxybergamottin
to the effects of grapefruit juice on cyclosporine
disposition and to assess the role of CYP3A4 versus P-glycoprotein in
this interaction. METHODS: The disposition of oral
cyclosporine was compared in healthy subjects after ingestion of
water,
grapefruit juice, and Seville orange juice. Enterocyte
concentrations of CYP3A4 were measured in 2 individuals before and
after
treatment with Seville orange juice. The effect of
6',7'-dihydroxybergamottin on P-glycoprotein was assessed in vitro.
RESULTS: Area under the whole blood
concentration-time curve and peak concentration of cyclosporine were
increased by 55% and 35%, respectively, with
grapefruit juice (P < .05). Seville orange juice had no influence on
cyclosporine disposition but reduced enterocyte
concentrations of CYP3A4 by an average of 40%.
6',7'-Dihydroxybergamottin did not inhibit P-glycoprotein at
concentrations
up to 50 micromol/L. CONCLUSIONS: 6',7'-Dihydroxybergamottin is not
responsible for the effects of grapefruit juice on
cyclosporine. Because the interaction did not occur with Seville
orange
juice despite reduced enterocyte concentrations of
CYP3A4, inhibition of P-glycoprotein activity by other compounds in
grapefruit juice may be responsible. Reduced enterocyte
CYP3A4 by 6',7'-dihydroxybergamottin could be important for other
drugs
whose bioavailability is less dependent on
P-glycoprotein.
***********************
Anyone see what i see? Try this one at home. Eat or drink a bunch
of grapefruits or juice. Eat a diet with high arachidonics. Egg
yolks and bacon (any pork) and deserts in close proximity. Or
any sugary foods. Sweet and Sour pork. You get the idea.
In other words get your P to flare. Then see if St. Johns wort will
stop it. I know that grapefruit juice induces (thru the p450) a major
flare in about a week of eating or drinking it.
You should get to know your triggers. I mean food here.
Keyword CYP3A4 P-450 randall (in the P ng)
randall... playing with p can be eye opening. See P grow and snow. Now stop it.
**********************************************************************