New natural Sweetner----Luo Han Guo Extract and new natural juice-------Luo Han Guo Juice
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with sugar. It is 250 times sweeter than sugar. It is also good for
body. Here we post some chemisty and function introduction. If you need
more picture, TLC and HPLC picture, please contact with us by mail.
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Luo Han Guo extract (Momordica Grosvenori P.E.) is mainly composed of
triterpenoid mogrosides, amino acids and vitamin, etc.
Triterpenoid glycosides
The glycosides in Luo Han Guo mainly as triterpene glycosides，part of
compounds of cucurbitane type, the collecting of saponins. It includes
Mogrosides II, III, IV, V, VI, siamcroside I, 11-oxo-Mogroside and
neomogroside, etc. Triterpenoid glycoside V in Luo Han Guo is the major
composition for sweetener.
Mogroside V
Glc＝ β－D－glucopyranosyl
C60H102O29·2H2O ； 1323.47
(white
powder，mp196～198℃。IRνmax(KBr)cm-1：3362.6（OH），1643.5（C-C）。1H-NMR〔1〕。13C-NMR。Sweetness:425times
than 5％saccharose（0.012％，H2O）。
Amino acids
With complete 18 kinds of basic amino acids, but tryptophan undetected,
Luo Han Guo also contains protein, including coarse & pure protein.
Item Result
ASP 3.08%
SER 2.18%
GLU 2.02%
GLY 1.54%
HIS 0.44%
ARG 1.27%
THR 1.27%
ALA 1.48%
PRO 2.04%
CYS 0.26%
TYR 1.44%
VAL 1.60%
MET 0.29%
LYS 0.81%
ILE 1.46%
LEU 1.85%
BHE 1.44%
TOTAL Amino acid 24.5%
TOTAL protein 26.2%
Assay data from The Center For Agri-food Quality & Safety,The ministry
of Agriculture,PRC
Vitamin Plentiful vitamin C with content 339.68-487.52mg/100g exists
in the fruit of Luo Han Guo in mature, along with 24 kinds of inorganic
elements as well, among which 16 kinds act as necessary trace elements
for human body. In addition, there are 27-33% of fat in the kernel
of Luo Han Guo. The necessary fat acids for human body add up to
linoleic acid, oleic acid, palmic acid, covering 7.7% of its kind.
D-mannitol also exists in fresh fruit.
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efficacy of Siraitia Grosvenori powder (SGP) and its extracts (SGE) on
blood glucose in normal and diabetic mice have been studied. The result
is bellowed:
4.1 Compare the body weight's variety before test to that after test
4.1.1 Effect of SGP and SGE on weight of normal mice
Table 1 Effect of SGP and SGE on weight of normal mice
Group Dose
(g/kg.bw) n Weight
Test-prophase (g) Test-metaphase (g) Test-anahase (g)
Control group
(Normal mice) 0 10 20.1±0.7 25.2±1.0 30.2±1.2
Mice i.g. SGP 0.5 10 20.2±0.6 26.0±1.4 30.9±1.5
1.0 10 20.1±0.9 25.6±0.8 30.2±1.0
3.0 10 20.0±0.8 26.3±1.4 31.3±1.7
Mice i.g. SGE 0.5 10 20.6±1.7 25.9±1.5 29.8±1.4
1.0 10 21.2±1.3 26.6±1.1 30.2±1.2
3.0 10 20.1±1.4 23.9±1.3 29.8±1.6
table 1 showed that whatever in Test-prophase or Test-metaphase or
Test-anahase all group mice's body weight were not different
distinctly comparing to the control group.
4.1.2 Effect of SGP and SGE on weight of alloxan-induced diabetic mice
Table 2 Effect of SGP and SGE on weight of alloxan-induced diabetic
mice
Group Dose
g/kg.bw) n Weight
Test-prophase (g) Test-metaphase (g) Test-anahase (g)
Control group
(Diabetic mice) 0 10 20.6±0.7 21.4±0.8 23.2±0.6
Mice i.g. SGP 0.5 10 20.3±1.0 21.8±1.8 23.6±1.6
1.0 10 20.4±1.2 22.0±0.8 23.9±1.2
3.0 10 20.3±0.8 22.1±1.1 23.5±1.1
Mice i.g. SGE 0.5 10 20.5±1.6 21.2±1.4 22.2±2.3
1.0 10 21.0±1.7 21.4±1.3 23.3±2.3
3.0 10 20.7±1.5 22.4±1.3 22.6±1.4
Table 2 showed that whatever in Test-prophase or Test-metaphase or
Test-anahase all group mice's body weight were not different
distinctly comparing to the control group.
4.2 Effect of SGP and SGE on the blood glucose level of normal mice
Table 3 Effect of SGP and SGE on the fasting blood glucose level of
normal mice
Group Dose
(g/kg.bw) n Blood Glucose
(mmol/L)
Control group
(Normal mice) 0 10 5.15±01.36
Mice i.g. SGP 0.5 10 5.45±0.77
1.0 10 4.84±1.20
3.0 10 4.28±1.67
Mice i.g. SGE 0.5 10 5.69±1.09
1.0 10 5.61±0.88
3.0 10 5.39±1.05
table 3 showed that whatever in the forepart period or mid-part period
or at the end of test all group mice's fasting blood glucose were not
different distinctly comparing to the control group.
4.2.2 Effect of SGP and SGE on the oral glucose tolerance of normal
mice
Table 4 Effect of SGP and SGE on the oral glucose tolerance of normal
mice
Group Dose
(g/kg.bw) n Glucose
(g/kg.bw) Blood Glucose(mmol/L)
0h 0.5h 2h
Control group
(Normal mice) 0 10 2.5 5.15±1.36 8.03±2.17 7.99±3.21
Mice i.g. SGP 0.5 10 2.5 5.45±0.77 10.05±2.51 7.80±1.16
1.0 10 2.5 4.84±1.20 9.55±3.85 7.57±2.30
3.0 10 2.5 4.28±1.67 7.37±2.09 6.84±1.43
Mice i.g. SGE 0.5 10 2.5 5.69±1.09 9.61±2.49 8.28±1.00
1.0 10 2.5 5.61±0.88 7.24±1.99 6.38±1.73
3.0 10 2.5 5.39±1.05 7.82±1.59 7.76±2.46
table 4 showed that whatever in the forepart period or mid-part period
or at the end of test all group mice's blood glucose were not
different distinctly comparing to the control group.
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diabetic mice
4.3.1 Effect of SGP and SGE on the fasting blood glucose of
alloxan-induced diabetic mice
Table 5 Effect of SGP and SGE on the fasting blood glucose of
alloxan-induced diabetic mice
Group Dose
Before test (g) After test (g)
Control group
(Diabetic mice) 0 10 15.42±5.75 19.58±1.58
Mice i.g. SGP 0.5 10 15.56±5.86 18.66±5.43
1.0 10 15.54±5.83 17.05±5.18
3.0 10 15.61±5.87 15.49±5.72*
Mice were i.g. SGE 0.5 10 15.45±5.76 11.84±4.24**
1.0 10 15.52±5.70 15.08±4.64*
3.0 10 15.54±5.77 20.35±4.36
"*" compared to diabetic mice, P<0.05, "**" compared to
diabetic control, P<0.01
Table 5 showed that the fasting blood glucose of the high dosage SGP
group, that of the mid-dosage SGE group and that of low-dosage SGE
group were all lower than the control group. low-dosage SGE have the
best effect on decreasing blood glucose. The result indicate that SGP
at dosage of 3 g/kg.bw and SGE at the dosage of 0.5~1.0 g/kg.bw are all
have the effect on decreasing blood glucose.
4.3.2 Effect of SGP and SGE on the oral glucose tolerance of
alloxan-induced diabetic mice
Table 6 Effect of SGP and SGE on the oral glucose tolerance of
alloxan-induced diabetic mice
Group Dose
Mice i.g. SGP 0.5 10 2.5 18.66±5.43 31.88±5.60 24.88±5.32
1.0 10 2.5 17.05±5.18 29.63±3.79 23.32±3.48
3.0 10 2.5 15.49±5.72* 28.89±5.37* 23.37±4.80
Mice i.g. SGE 0.5 10 2.5 11.84±4.24** 28.60±2.18* 22.45±5.78
1.0 10 2.5 15.08±4.64* 33.00±4.89 27.77±5.30
3.0 10 2.5 20.35±4.36 33.25±5.14 25.34±5.14
"*" compared to diabetic mice, P<0.05, "**" compared to
diabetic control, P<0.01
Table 6 showed that comparing the control group, the blood glucose of
mice i.g. high dosage SGP after 0.5h is lower. the blood glucose of
mice i.g. low dosage SGE after 0h and 0.5h is lower as well.
The above text indicated Siraitia Grosvenori powder(SGP) and its
extracts(SGE) have the effect on decreasing blood glucose distinctly.
Comparing to the contract group (High blood glucose mice) its active in
preventing blood glucose lelve is outstanding. It showed that Siraitia
grosvenori and its extracts can weaken the damage of pancreatic B cell
induced by alloxan and improve the function of damaged cell. It is
benefit to relive the Diabetic symptom. The low dosage (0.5 g/kg.bw
)SGE (containing 80% mogrosides) was prior to high dosage SGP(3.0
g/kg.bw).It is probably proved that triterpene component mogrosides may
be the active ingredients.
It is reported that the decreasing blood glucose function of mogrosides
is possible relative to its own anti-oxidant. Antioxidant can decrease
the free radical, thus is good for control the diabetic. Furthermore,
it have antimicrobial and immune nodulation functions, which can
prevent the diabetic complication, the further research on it is keen
on.
Mogrosides is a natural product, which is of high saccharinity, low
calorie, innocuous and safety. The result of the test showed that
mogrosides have effect on normal mice, which is different from anther
decreasing blood g products. Therefore, using mogrosides as sweetening
reagent for healthy drink or decreasing blood glucose glucose drugs are
all promising.