Campus Stem Cell Lab meeting, Tuesday, April 16
Sue Gilbert
Our next talk this semester will be on Tuesday, April 16, and will start at noon promptly. We will continue to meet in The Wisconsin Institutes of Discovery (WID) for most of the talks. We will be meeting in the H.F. Deluca Forum in the Town Center on the first floor. Please ask at Information Desk if you are unsure where to go. We will continue to provide sandwiches and chips starting at 11:50am (water will be provided but if you want something else to drink please bring your own.)
Date: Tuesday, April 16
Speaker: Kran Suknuntha, Research Assistant, Cellular and Molecular Pathology (Slukvin lab)
Title: Generation of Leukemic stem cell-like cells from iPSCs derived from Chronic Myeloid Leukemia
Abstract: The definitive cure of leukemia requires identification of novel therapeutic targets to eradicate leukemia stem cells (LSCs). However, rarity of LSCs within the pool of malignant cells remains major limiting factor for their study in humans. Here we show that lin-CD34+CD45+ cells with LSC features can be generated from induced pluripotent stem cells (iPSCs) derived from chronic myeloid leukemia (CML) patient. Similar to parental somatic LSCs, CML-iPSCs-derived lin-CD34+CD45+ cells were resistant to thyrosine kinase inhibitor (TKI) imatinib, but became sensitive after maturation. Comparative analysis of gene expression in CML-iPSCs-derived lin-CD34+CD45+ cells treated and not treated with imatinib identified olfactomedin 4 (OLFM4) as the top-ranking gene induced by TKI. OLFM4 knockdown using siRNA induced apoptosis and inhibited growth in CML-iPSCs-derived lin-CD34+CD45+ cells and somatic LSCs isolated from parental and unrelated bone marrow samples. Overall, our studies demonstrated that iPSC-based model could be used successfully to interrogate LSCs and identify novel drug targets.
Reminder: If you are talking this Seminar Series for credit, please check in with Sue Gilbert at the back of the room.
Next talk: On Tuesday, April 23, our speaker will be Daniel DiRenzo, Postdoctoral Trainee (Kent Lab), Vascular Surgery, who will be speaking on “TGF-beta/SMAD3 Driven Smooth Muscle Cell De-differentiation.” This talk will be in Wisconsin Institutes of Discovery (WID), H.F. Deluca Forum in the Town Center on the first floor, and will include sandwiches.
A list of the talks for the semester is found at:
http://stemcells.wisc.edu/node/246
Thank you for supporting this venue for the sharing of information.
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Sue Gilbert
Office Operations Associate
Stem Cell and Regenerative Medicine Center
5036 WIMR
1111 Highland Avenue
Madison, WI 53705-2275
Tel: (608) 263-2982
FAX: (608) 263-5267
"The science fiction of yesterday happens to be a lot of the medicine of today"