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trapper fails when large files processed
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Ilya.Agron  
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 More options Jul 2, 3:26 am
From: "Ilya.Agron" <ilya.ag...@gmail.com>
Date: Thu, 2 Jul 2009 00:26:02 -0700 (PDT)
Local: Thurs, Jul 2 2009 3:26 am
Subject: trapper fails when large files processed
There's a question about the trapper in TPP (Win-version).
Now we use trapper to convert .d (Agilent-files, Q-ToF) in mzXML; and
then use Decon2ls to process it into deconvoluted and deisotoped data
for AMT approach. It's our work flow very shortly.
There's problem, that we can't process with trapper large files (as
our 4.5 Gb chromatograms). It fails during the processing. Is it the
problem of the memory?
May you advise something to solve this problem?
Thanks in advance!

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David Shteynberg  
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 More options Jul 7, 12:16 pm
From: David Shteynberg <dshteynb...@systemsbiology.org>
Date: Tue, 7 Jul 2009 09:16:52 -0700
Local: Tues, Jul 7 2009 12:16 pm
Subject: Re: [spctools-discuss] trapper fails when large files processed
Hello Ilya,

I have not used trapper but in a situation like this a more detailed
description of the failure may be needed.  Are there any error
messages produced? What is the system you are running trapper on?
What else have to tried to address this issue?  Can you post a problem
file to Tranche and share the key so we can try to reproduce the
problem here?

-David


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Ilya.Agron  
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 More options Jul 8, 8:32 am
From: "Ilya.Agron" <ilya.ag...@gmail.com>
Date: Wed, 8 Jul 2009 05:32:26 -0700 (PDT)
Local: Wed, Jul 8 2009 8:32 am
Subject: Re: trapper fails when large files processed
Hello.

The last time I've used it the error looked this way:

E:\trapper>trapper.exe --mzXML -c E:\trapper\MasKate1-r001.d
single mode file
(got computer name: RIGHT)
ERROR at .\MHDACWrapper.cpp, 227:
HR = -2147024882
error getting spectrum
empty scan object

E:\trapper>

What could it be?
May be, it's not memory problem. There's Windows OS.

What's Tranche? Is it free upload resource?
Sure, I can and want to upload it.

Thanks!

On Jul 7, 8:16 pm, David Shteynberg <dshteynb...@systemsbiology.org>
wrote:


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David Shteynberg  
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 More options Jul 20, 2:00 pm
From: David Shteynberg <dshteynb...@systemsbiology.org>
Date: Mon, 20 Jul 2009 11:00:56 -0700 (PDT)
Local: Mon, Jul 20 2009 2:00 pm
Subject: Re: trapper fails when large files processed
Hello Ilya,

The error explains the problem "error getting spectrum
empty scan object".   The error is referring to the follow bit of
code:

  215 MH::BDA::IBDASpecData* MHDACWrapper::GetSpectrum
(MH::IMsdrDataReader* pMSDataReader,
  216                                                                                            double retentionTime,
  217                                                                                            MH::MSScanType scanType,
  218                                                                                            MH::IonPolarity ionPolarity,
  219                                                                                            MH::IonizationMode ionizationMode,
  220                                                                                            MH::IMsdrPeakFilter* filter,
  221                                                                                            VARIANT_BOOL* filterOnCentroid)
  222 {
  223   MH::BDA::IBDASpecData* specData = NULL;
  224   COMCHECK(pMSDataReader->GetSpectrum_7(
  225           retentionTime,
  226           scanType,
  227           ionPolarity,
  228           ionizationMode,
  229           filter,
  230           *filterOnCentroid,
  231           &specData),
  232           "error getting spectrum");
  233
  234   return specData;
  235 }

The failure is on a COM object call to an Agilent software library
function call.  Are you sure there is actual data in the file you are
giving to trapper to convert? Have you opened this data file in an
agilent viewer? Would you mind posting your raw data to our FTP site
(instructions here
http://tools.proteomecenter.org/wiki/index.php?title=TPP:Frequently_A...
We can then try to reproduce your problem in a debugger to find the
cause of failure.

-David

On Jul 8, 5:32 am, "Ilya.Agron" <ilya.ag...@gmail.com> wrote:


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