Hi everyone,
is there a way in the TPP, to filter a .pep.xml file in other to keep ONLY the hit with the highest score ?
As in the example below I would like to eliminated all the other peptide identifications except teh one with the highest score.
thanks
alessio
:
/PeptideIdentification>
<PeptideIdentification score_type="xcorr" higher_score_better="true" significance_threshold="0" MZ="
609.8572998" RT="2549.54" >
<PeptideHit score="1" sequence="LISQIVSSITAS" charge="2" aa_before="R" aa_after="L" protein_refs="PH_1087" >
</PeptideHit>
<PeptideHit score="1.6019" sequence="LLLLLPPT(Phospho)LM(Oxidation)" charge="2" aa_before="P" aa_after="-" protein_refs="PH_2478" >
</PeptideHit>
<PeptideHit score="1.5255" sequence="LLERAGTLSMK" charge="2" aa_before="P" aa_after="P" protein_refs="PH_11293" >
</PeptideHit>
<PeptideHit score="1.5115" sequence="LLLWGASM(Oxidation)GVR" charge="2" aa_before="E" aa_after="R" protein_refs="PH_11294" >
</PeptideHit>
<PeptideHit score="1.488" sequence="ILGALPC(Carbamidomethyl)SM(Oxidation)LK" charge="2" aa_before="S" aa_after="K" protein_refs="PH_2960" >
</PeptideHit>
<PeptideHit score="1.4504" sequence="ILESTTPGAAFL" charge="2" aa_before="K" aa_after="L" protein_refs="PH_11295" >
</PeptideHit>
<PeptideHit score="1.4178" sequence="LIKLC(Carbamidomethyl)LSRC(Carbamidomethyl)G" charge="2" aa_before="K" aa_after="S" protein_refs="PH_11296" >
</PeptideHit>
<PeptideHit score="1.3869" sequence="LLKNDANFRK" charge="2" aa_before="L" aa_after="L" protein_refs="PH_11297" >
</PeptideHit>
<PeptideHit score="1.3633" sequence="LLQTQRELMS" charge="2" aa_before="R" aa_after="K" protein_refs="PH_11298" >
</PeptideHit>
<PeptideHit score="1.3605" sequence="LLQLPRT(Phospho)AVK" charge="2" aa_before="S" aa_after="S" protein_refs="PH_11299" >
</PeptideHit>
</PeptideIdentification>