solexa

Typically a good HiSeq fully processed run where you have the FASTQ files generated will range from

On a GA, no. It's much more likely to work on a HiSeq, though as you say, it's not a great

What kind of modifications/purifications did you get for your adapter sequences that were synthesized

I use R package SPP for chip-seq analysis. It can read quite a few aligner's output file. Here is

Thank you for the responses. In our case, A 100PE flowcell takes about 6 days of GERALD run time(

Labindia-GPOD is organizing aa 7 day workshop on Next-Gen Sequencing Data Analysis at Rajiv Gandhi

You may want to check out Sample Tracking in Galaxy. It has a neat data transfer mechanism, where a

index_decoder is just the first step in the process if you want separate files. The sequenceread

We are trying to produce a cDNA library for RNA-Seq from 100ng of total RNA using the DSN

Hi Hemant, Thanks a lot Anna On 8 Sep, 13:07, hemant kelkar <hkel...@gmail.com> wrote: > Hi

Hi all, I am wondering that any options for generating scores (QC, NM,U0,U1,U2, R0,R1,R2) and x,y,z(

Hi Lingsheng, Thanks for response. As far as I know s_N_export.txt file contains the results of

Exactly right! Illumina has done nobody any favors with regards to bcl files. Bcl files had always

Are you using ELAND_SET_SIZE in your Gerald config files? From the documentation: The maximum number

Switching ciphers will help, but only a little (a few %, at most). You're better off using a

To follow-up on what I wrote, we have 1 GA and 1 HiSeq and currently do only QA/QC alignment. We are

Hi Gustavo, On Jul 27, 2010, at 12:23 AM, Gustavo Kijak wrote: > A little bit of background: >

Hi , Happy Earth Day! Our planet is facing more trouble with every passing year—from the climate

If you google oligo-dT hybridization buffer, you should be able to find the buffer for hybridization.

Leath, We have found that one can run demultiplexGerald jobs in "parallel" in all 12

Planned use of microarrays in 2010 compared to 2009 is expected to be: Upregulated / DownRegulated /

Dear Colleagues, We would like to request your participation in the poll created at Linkedin to get

Hi, I would like to know if someone have tried to map WGS Illumina reads from one species to a

Sounds good Chris. I was not aware that default moderation is on for new users. Cheers, -Abhi On Fri,

Dear Solexa, Oops!! I forgot to invite you to Just Dial's Lucky Referrer Contest. The last

dear all, Has anyone ever used the padlock capture to select regions for the next sequencing? thanks

On Phred scale.. I would say it starts around 33-36 and then depending on the length of reads(cycles)

Hi, Check the following article. Transcript length bias in RNA-seq data confounds systems biology

I will be out of the office starting 01/07/2010 and will not return until 01/15/2010. I will respond

Dear All, May The Year 2010 Bring for You Happiness, Success and filled with Peace,Hope &