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Lingsheng Dong
, …
Abhishek Pratap
3
14.12.2010
HiSeq data size
Typically a good HiSeq fully processed run where you have the FASTQ files generated will range from
nieprzeczytany,
HiSeq data size
Typically a good HiSeq fully processed run where you have the FASTQ files generated will range from
14.12.2010
Sivakumar Gowrisankar
, …
J
7
16.11.2010
Removing flowcell between two reads
On a GA, no. It's much more likely to work on a HiSeq, though as you say, it's not a great
nieprzeczytany,
Removing flowcell between two reads
On a GA, no. It's much more likely to work on a HiSeq, though as you say, it's not a great
16.11.2010
sherisim
,
elaneyk
2
9.11.2010
problems with cloned library molecules
What kind of modifications/purifications did you get for your adapter sequences that were synthesized
nieprzeczytany,
problems with cloned library molecules
What kind of modifications/purifications did you get for your adapter sequences that were synthesized
9.11.2010
nacoo2000
, …
Lingsheng Dong
3
28.10.2010
How can I ideal with the next generation sequencing in R or bioconductor environment
I use R package SPP for chip-seq analysis. It can read quite a few aligner's output file. Here is
nieprzeczytany,
How can I ideal with the next generation sequencing in R or bioconductor environment
I use R package SPP for chip-seq analysis. It can read quite a few aligner's output file. Here is
28.10.2010
yongmei zhao
, …
Sivakumar Gowrisankar
4
18.10.2010
HiSeq data processing with CASAVA1.7
Thank you for the responses. In our case, A 100PE flowcell takes about 6 days of GERALD run time(
nieprzeczytany,
HiSeq data processing with CASAVA1.7
Thank you for the responses. In our case, A 100PE flowcell takes about 6 days of GERALD run time(
18.10.2010
uday
16.10.2010
Next-Gen Sequencing Data Analysis Workshop
Labindia-GPOD is organizing aa 7 day workshop on Next-Gen Sequencing Data Analysis at Rajiv Gandhi
nieprzeczytany,
Next-Gen Sequencing Data Analysis Workshop
Labindia-GPOD is organizing aa 7 day workshop on Next-Gen Sequencing Data Analysis at Rajiv Gandhi
16.10.2010
Sivakumar Gowrisankar
, …
rc
11
8.10.2010
Data delivery for HiSeq
You may want to check out Sample Tracking in Galaxy. It has a neat data transfer mechanism, where a
nieprzeczytany,
Data delivery for HiSeq
You may want to check out Sample Tracking in Galaxy. It has a neat data transfer mechanism, where a
8.10.2010
Sivakumar Gowrisankar
, …
Kevin M. Carr
4
4.10.2010
Demultiplexing slow
index_decoder is just the first step in the process if you want separate files. The sequenceread
nieprzeczytany,
Demultiplexing slow
index_decoder is just the first step in the process if you want separate files. The sequenceread
4.10.2010
Abdul K Sesay
9.09.2010
DSN normalisation
We are trying to produce a cDNA library for RNA-Seq from 100ng of total RNA using the DSN
nieprzeczytany,
DSN normalisation
We are trying to produce a cDNA library for RNA-Seq from 100ng of total RNA using the DSN
9.09.2010
Anna
,
hemant kelkar
3
8.09.2010
casava 1.7
Hi Hemant, Thanks a lot Anna On 8 Sep, 13:07, hemant kelkar <hkel...@gmail.com> wrote: > Hi
nieprzeczytany,
casava 1.7
Hi Hemant, Thanks a lot Anna On 8 Sep, 13:07, hemant kelkar <hkel...@gmail.com> wrote: > Hi
8.09.2010
Anna
18.08.2010
eland alignment file
Hi all, I am wondering that any options for generating scores (QC, NM,U0,U1,U2, R0,R1,R2) and x,y,z(
nieprzeczytany,
eland alignment file
Hi all, I am wondering that any options for generating scores (QC, NM,U0,U1,U2, R0,R1,R2) and x,y,z(
18.08.2010
Anna
,
Lingsheng Dong
3
18.08.2010
Eland alignment file
Hi Lingsheng, Thanks for response. As far as I know s_N_export.txt file contains the results of
nieprzeczytany,
Eland alignment file
Hi Lingsheng, Thanks for response. As far as I know s_N_export.txt file contains the results of
18.08.2010
Sivakumar Gowrisankar
, …
Bruce
9
13.08.2010
HiSeq data archive
Exactly right! Illumina has done nobody any favors with regards to bcl files. Bcl files had always
nieprzeczytany,
HiSeq data archive
Exactly right! Illumina has done nobody any favors with regards to bcl files. Bcl files had always
13.08.2010
Andrew Gagne
, …
Leath Tonkin
5
13.08.2010
HiSeq run times
Are you using ELAND_SET_SIZE in your Gerald config files? From the documentation: The maximum number
nieprzeczytany,
HiSeq run times
Are you using ELAND_SET_SIZE in your Gerald config files? From the documentation: The maximum number
13.08.2010
Aparna P
, …
Jesse Becker
9
10.08.2010
wondering how everybody operates the GA pipeline
Switching ciphers will help, but only a little (a few %, at most). You're better off using a
nieprzeczytany,
wondering how everybody operates the GA pipeline
Switching ciphers will help, but only a little (a few %, at most). You're better off using a
10.08.2010
Andrew Gagne
, …
Sivakumar Gowrisankar
5
27.07.2010
Size of IT/Bioinformatics team
To follow-up on what I wrote, we have 1 GA and 1 HiSeq and currently do only QA/QC alignment. We are
nieprzeczytany,
Size of IT/Bioinformatics team
To follow-up on what I wrote, we have 1 GA and 1 HiSeq and currently do only QA/QC alignment. We are
27.07.2010
Gustavo Kijak
, …
Davide Cittaro
3
27.07.2010
Can someone recommend a consultant to help us integrate our high performance computing and storage infrastructure in support of Illumina NGS?
Hi Gustavo, On Jul 27, 2010, at 12:23 AM, Gustavo Kijak wrote: > A little bit of background: >
nieprzeczytany,
Can someone recommend a consultant to help us integrate our high performance computing and storage infrastructure in support of Illumina NGS?
Hi Gustavo, On Jul 27, 2010, at 12:23 AM, Gustavo Kijak wrote: > A little bit of background: >
27.07.2010
mohits...@gmail.com
23.04.2010
Happy Earth Day
Hi , Happy Earth Day! Our planet is facing more trouble with every passing year—from the climate
nieprzeczytany,
Happy Earth Day
Hi , Happy Earth Day! Our planet is facing more trouble with every passing year—from the climate
23.04.2010
PeroMHC
,
Stanley Han
2
18.04.2010
sera-mag bead buffers for mRNA library?
If you google oligo-dT hybridization buffer, you should be able to find the buffer for hybridization.
nieprzeczytany,
sera-mag bead buffers for mRNA library?
If you google oligo-dT hybridization buffer, you should be able to find the buffer for hybridization.
18.04.2010
Sivakumar Gowrisankar
, …
hemant kelkar
6
16.04.2010
CASAVA 1.6 GERALD
Leath, We have found that one can run demultiplexGerald jobs in "parallel" in all 12
nieprzeczytany,
CASAVA 1.6 GERALD
Leath, We have found that one can run demultiplexGerald jobs in "parallel" in all 12
16.04.2010
NextGenSeq
2.04.2010
Future of Microarrays in Next Gen Sequencing (NGS) Era?
Planned use of microarrays in 2010 compared to 2009 is expected to be: Upregulated / DownRegulated /
nieprzeczytany,
Future of Microarrays in Next Gen Sequencing (NGS) Era?
Planned use of microarrays in 2010 compared to 2009 is expected to be: Upregulated / DownRegulated /
2.04.2010
NextGenSeq
13.02.2010
Solexa Illumina Vs SOLiD Poll and results
Dear Colleagues, We would like to request your participation in the poll created at Linkedin to get
nieprzeczytany,
Solexa Illumina Vs SOLiD Poll and results
Dear Colleagues, We would like to request your participation in the poll created at Linkedin to get
13.02.2010
João Fadista
10.02.2010
align to different ref assembly
Hi, I would like to know if someone have tried to map WGS Illumina reads from one species to a
nieprzeczytany,
align to different ref assembly
Hi, I would like to know if someone have tried to map WGS Illumina reads from one species to a
10.02.2010
Abhishek Pratap
,
Chris Seidel
3
22.01.2010
Please moderate messages on this group
Sounds good Chris. I was not aware that default moderation is on for new users. Cheers, -Abhi On Fri,
nieprzeczytany,
Please moderate messages on this group
Sounds good Chris. I was not aware that default moderation is on for new users. Cheers, -Abhi On Fri,
22.01.2010
Praveen
22.01.2010
Hey Solexa, I forgot to mention...
Dear Solexa, Oops!! I forgot to invite you to Just Dial's Lucky Referrer Contest. The last
nieprzeczytany,
Hey Solexa, I forgot to mention...
Dear Solexa, Oops!! I forgot to invite you to Just Dial's Lucky Referrer Contest. The last
22.01.2010
quarksu
12.01.2010
select region
dear all, Has anyone ever used the padlock capture to select regions for the next sequencing? thanks
nieprzeczytany,
select region
dear all, Has anyone ever used the padlock capture to select regions for the next sequencing? thanks
12.01.2010
Scott Coutts
,
Abhishek Pratap
2
11.01.2010
Average Q value
On Phred scale.. I would say it starts around 33-36 and then depending on the length of reads(cycles)
nieprzeczytany,
Average Q value
On Phred scale.. I would say it starts around 33-36 and then depending on the length of reads(cycles)
11.01.2010
Victor Ruotti
, …
Ravi Gupta
3
8.01.2010
Short genes vs. long genes expression
Hi, Check the following article. Transcript length bias in RNA-seq data confounds systems biology
nieprzeczytany,
Short genes vs. long genes expression
Hi, Check the following article. Transcript length bias in RNA-seq data confounds systems biology
8.01.2010
Richard Cronn
7.01.2010
Richard Cronn/PNW/USDAFS is out of the office.
I will be out of the office starting 01/07/2010 and will not return until 01/15/2010. I will respond
nieprzeczytany,
Richard Cronn/PNW/USDAFS is out of the office.
I will be out of the office starting 01/07/2010 and will not return until 01/15/2010. I will respond
7.01.2010
Hersh Parikh
31.12.2009
Happy New Year
Dear All, May The Year 2010 Bring for You Happiness, Success and filled with Peace,Hope &
nieprzeczytany,
Happy New Year
Dear All, May The Year 2010 Bring for You Happiness, Success and filled with Peace,Hope &
31.12.2009