Proteomics with a triple quad?

[DougBen...@googlemail.com]

Good day everyone,

Is there a relationship between the number of amino acids in an
oligomer and the fragmentation efficiency in a low energy CID MS? For
example, it is my understanding that large proteins cannot be
fragmented in triple quad or ion trap collision cells because there
are too many degrees of freedom in bond rotations, so CID energy would
be dispersed through rotation and other processes; thus necessitating
other techniques such as ECD and IRMPD. But for bottom up proteomic
work, is there a limitation at which point the oligomers become too
big to fragment inside a low energy collision cell?

Thank you very much for your consideration and patience with my
rudimentary question.

Cheerio,
Doug