Google Groups no longer supports new Usenet posts or subscriptions. Historical content remains viewable.
Dismiss
peptide analysis by MicroTOF (Bruker)
1 view
Skip to first unread message
Lhin Chen
Mar 20, 2008, 1:38:05 AM3/20/08
to
Hi all
I am using MicroTOF (Bruker) for detection of unknown peptide masses. I
found 5 series of masses (m/z 988-1016 Da with 7 Da different in each
compound). Can they be defined as doubly charge ions? This is because the
calculation indicated mono-isotopes of 14 Da interval from each mass
where it is possible to be CH2 group added in to the peptide chain.
However, I could not observe singly charge peak. One reason to support
the doubly charge hypothesis is MALDI-TOF showed mass spectrum range from
1900-2000 Da.
Does anyone have a suggestion ?
Thank you in advance
Chip Cody
Mar 20, 2008, 10:43:38 AM3/20/08
to
If the peptides have molecular weights near 2000, you should have more
than enough resolving power with a TOF to be able to observe the
isotope peaks for the double charge ions. The isotope peaks should be
spaced at m/z intervals of 1 fo rsingle charge ions, 0.5 for double
charge ions, 0.33 for triple charge ions, etc.
than enough resolving power with a TOF to be able to observe the
isotope peaks for the double charge ions. The isotope peaks should be
spaced at m/z intervals of 1 fo rsingle charge ions, 0.5 for double
charge ions, 0.33 for triple charge ions, etc.
0 new messages